Protein Info for AO353_13570 in Pseudomonas fluorescens FW300-N2E3

Annotation: TonB-dependent receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 709 signal peptide" amino acids 1 to 45 (45 residues), see Phobius details TIGR01778: TonB-dependent copper receptor" amino acids 74 to 709 (636 residues), 966.7 bits, see alignment E=3.5e-295 PF07715: Plug" amino acids 75 to 173 (99 residues), 49.9 bits, see alignment E=4.1e-17 PF00593: TonB_dep_Rec_b-barrel" amino acids 227 to 667 (441 residues), 168.9 bits, see alignment E=3.6e-53

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 87% identity to pfo:Pfl01_0596)

Predicted SEED Role

"Outer membrane receptor proteins, mostly Fe transport" in subsystem Hemin transport system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VPN2 at UniProt or InterPro

Protein Sequence (709 amino acids)

>AO353_13570 TonB-dependent receptor (Pseudomonas fluorescens FW300-N2E3)
MSRFSADTRLSAAQAFFALNAPRARFKQATAILCGALLSPLASAAEQPVDHSQHTEELSP
TVITAIAPSSPLTIVTNPKDPRQPVPASDGGDYLKTIPGFALVRNGGTNGDPVLRGMFGS
RLNILTNGGMILGACPGRMDAPTSYISPETYDKLTVIKGPQTVLWGPGASAGTILFDREP
ESFGELGTRVNASLLAGSNGRFDKLVDAAAGGPLGYIRVIGNTAHADDYRDGNNDTVPSR
YDKWNGDVALGWTPDADTLLELTAGKGDGEARSAGRGMDGSQFKRESLGLRFEKSNLGEV
LDKVEAQVYYNYADHVMDNYTLRTPSGTGMMAGPMASNVDRRTLGARIKATWRWADVQLI
SGIDAQTNEHRQRAAMGIDTYKDVPRNKDADFHNYGAFSELTWYAADRDRLISGARLDRA
SAKDFRQTIGSGMMSRPNPTVDKSRADTLPSGFVRYEHDLADSPTTLYAGLGHAERFPDY
WELFSPKSGPAGSLNAFDSIKPEKTTQLDFGLQYKTQDLEAWASGYVGEVHDYILFDYTP
GMMGMTSRAENINARIMGGELGAAYNLTSHWKADATLAYAWGKDTTHDTALPQMPPLDAR
FGLTYSEDNWSAGALWRVVAAQNRIEQNKGNVVGKDYDKSPGFGVFSLNGAYRIDKHWKV
STGVDNLFGKAYAEHLNLAGNAGFGYPANDPQAINEPGRTFWTKVDMSF