Protein Info for AO353_13440 in Pseudomonas fluorescens FW300-N2E3
Annotation: alpha/beta hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01046, triacylglycerol lipase [EC: 3.1.1.3] (inferred from 85% identity to pfo:Pfl01_0571)Predicted SEED Role
"Lipase precursor (EC 3.1.1.3)" in subsystem Cytolysin and Lipase operon in Vibrio (EC 3.1.1.3)
MetaCyc Pathways
- triacylglycerol degradation (2/3 steps found)
- retinol biosynthesis (2/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.3
Use Curated BLAST to search for 3.1.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9VPL1 at UniProt or InterPro
Protein Sequence (296 amino acids)
>AO353_13440 alpha/beta hydrolase (Pseudomonas fluorescens FW300-N2E3) MSQETSTRYPLVLVPGMLGFIRLLFYPYWYGIISALRRGGATVIAVQVSPVNSNEVRGEE LLVQIDEILRETGASKVNLIGHSQGSLTARYAAAKRPDRIASVTSVAGPNHGSELADYLH KHCPPNSAKGRVLSALLQLVAWLMGLLETGHPRHKWPVDVHASHDSLTSEGVALFNQRYP QGLPETWGGHGPELVNGVRYYSWSGTLQPGKTDRGGNLFDGTNRSCRLFATTFVREAGHC DGMVGRYSSHLGTVIGDEYPLDHFDIVNQSLGLVGKGADPLRLFVEHAQRLKAAGV