Protein Info for AO353_13035 in Pseudomonas fluorescens FW300-N2E3

Annotation: serine/threonine protein phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 61% identical to CPDA_PSEAI: 3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA (cpdA) from Pseudomonas aeruginosa

KEGG orthology group: K03651, Icc protein (inferred from 86% identity to pfo:Pfl01_0497)

Predicted SEED Role

"3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)" in subsystem cAMP signaling in bacteria (EC 3.1.4.17)

KEGG Metabolic Maps

Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.4.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H026 at UniProt or InterPro

Protein Sequence (271 amino acids)

>AO353_13035 serine/threonine protein phosphatase (Pseudomonas fluorescens FW300-N2E3)
LPSVSTLTANDAVLLVQLSDSHLFAEADGTLLGMNTRDSLQKVIELALAQQPRIDLVIAS
GDVSQDGTLESYQQFREMTRQIDAPARWIPGNHDEPQIMAQAAVQSQLLEPIVDIGNWRI
TLLDSAVPGSVPGYLQDDQLQLLARALSEAPERHHLVCLHHHPVSIGCAWMEPIGLRNPD
ALFAVLDRFPHVRAVLWGHVHQEIDQLRNDVRLIASPSTCIQFEPGSVDFKVDTLAPGYR
WLRLLPDGRLETGVERVTGFDFQVDYGSNGY