Protein Info for AO353_13010 in Pseudomonas fluorescens FW300-N2E3

Annotation: thiamine biosynthesis protein ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 24 to 101 (78 residues), 104.6 bits, E=1.9e-34
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 140 to 592 (453 residues), 719.2 bits, E=7.5e-221
• PF01964: ThiC_Rad_SAM
  • amino acids 142 to 590 (449 residues), 616.9 bits, E=1.8e-189

Best Hits

Swiss-Prot: 96% identical to THIC_PSEPF: Phosphomethylpyrimidine synthase (thiC) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 98% identity to pba:PSEBR_a512)

MetaCyc: 72% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See A0A0N9VUH5 at UniProt or InterPro

Protein Sequence (629 amino acids)

>AO353_13010 thiamine biosynthesis protein ThiC (Pseudomonas fluorescens FW300-N2E3)
MTTKSKNAINLSDSAKVDQQSVQPFTRSQKIYVQGSRPDILVPMREISLDVTPTDFGGEI
NAPVVVYDTSGPYTDPNVIIDVRKGLGDVRSPWIEARGDTERLAGLSSNFGQERLADPEL
TKLRFAHVNNPRRAKPGANVSQMHYARKGIITAEMEYVAIRENMKLEVARAAGLLDQQHA
GHSFGASVPKIITPEFVREEIARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALG
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGA
AEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCLAH
HKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADANDEAQFGELETLGELTKIAWKH
DVQTMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFE
FRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRI
ETVDAEVAQGLAEQAERFKQEGSQLYKKV