Protein Info for AO353_13005 in Pseudomonas fluorescens FW300-N2E3

Annotation: channel protein TolC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01844: type I secretion outer membrane protein, TolC family" amino acids 29 to 451 (423 residues), 414.1 bits, see alignment E=3.5e-128 PF02321: OEP" amino acids 35 to 218 (184 residues), 79.6 bits, see alignment E=1.4e-26 amino acids 243 to 436 (194 residues), 112.3 bits, see alignment E=1.3e-36

Best Hits

KEGG orthology group: K12340, outer membrane channel protein TolC (inferred from 92% identity to pfo:Pfl01_0491)

Predicted SEED Role

"Type I secretion outer membrane protein, TolC precursor" in subsystem Multidrug Resistance Efflux Pumps or Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WV54 at UniProt or InterPro

Protein Sequence (479 amino acids)

>AO353_13005 channel protein TolC (Pseudomonas fluorescens FW300-N2E3)
MLRKLSLALAVSCASNGMAWAAEAPLSSNTDLVSVYQEAVDNNADLAAARAQYGAQKEVV
PQARAGLLPNLSGSAEVADVRTKIDQPSAIANRSAHFYQATLAQPLFRADRWFQLQAAKD
VNEQAALQLSATEQNLILKSAETYFNVLRSQDNLASTKAEEAAFKRQLDQSNERFDVGLS
DKTDVLQSQASYDTARANRILAQRQVDDAFEALITLTNRQYNSIQGIVHTLPVLPPAPND
AKAWVDTAAKQNLNLLASNYAVSAAEETLKQRKAGHAPTLDAVAQYKTGDNDALGFSNPS
AFGQPYGGTVSQSTVGLQLNIPIYSGGLTSSQVRESYSRLDQNEQQREALRRQVVENTRN
LHRAVNSDVEQVQARRQSIISNQSAVEATEIGYQVGTRNIVDVLDAQRQLYTSVRNYNNS
RYDYILDNLRLKQAAGTLSPGDLQDLKRYLKADYNPDKDFLPPDLAKAAAEQLKANPGQ