Protein Info for AO353_12990 in Pseudomonas fluorescens FW300-N2E3

Annotation: multidrug DMT transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 110 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 31 to 51 (21 residues), see Phobius details amino acids 58 to 79 (22 residues), see Phobius details amino acids 85 to 104 (20 residues), see Phobius details PF00893: Multi_Drug_Res" amino acids 3 to 94 (92 residues), 92.6 bits, see alignment E=9.2e-31

Best Hits

Swiss-Prot: 62% identical to MTDTR_PSEAE: Multidrug transporter PA4990 (PA4990) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03297, small multidrug resistance protein, SMR family (inferred from 94% identity to pfl:PFL_0534)

MetaCyc: 42% identical to DLP12 prophage; multidrug/betaine/choline efflux transporter EmrE (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-344; TRANS-RXN0-493; TRANS-RXN0-532; TRANS-RXN0-533; TRANS-RXN0-628

Predicted SEED Role

"Ethidium bromide-methyl viologen resistance protein EmrE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WIM6 at UniProt or InterPro

Protein Sequence (110 amino acids)

>AO353_12990 multidrug DMT transporter (Pseudomonas fluorescens FW300-N2E3)
MNAYYLLAIAICAEVIATVSMKAVKGLSTPLPLLLIIVGYGIAFWMLTLVVRTIPVGVTY
AVWAGMGIVMVSIAALFLYDQKLDIPAMLGMALIVLGVVVIQLFSKTAGH