Protein Info for AO353_12970 in Pseudomonas fluorescens FW300-N2E3

Annotation: heptose 1-phosphate adenyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 TIGR02198: bifunctional protein RfaE, domain I" amino acids 5 to 312 (308 residues), 409.6 bits, see alignment E=1.3e-126 PF00294: PfkB" amino acids 13 to 301 (289 residues), 149.6 bits, see alignment E=2e-47 PF08543: Phos_pyr_kin" amino acids 187 to 286 (100 residues), 35.4 bits, see alignment E=1.2e-12 TIGR02199: bifunctional protein RfaE, domain II" amino acids 331 to 472 (142 residues), 213.7 bits, see alignment E=1.4e-67 TIGR00125: cytidyltransferase-like domain" amino acids 341 to 407 (67 residues), 58.8 bits, see alignment E=7e-20 PF01467: CTP_transf_like" amino acids 343 to 437 (95 residues), 67.2 bits, see alignment E=2.6e-22

Best Hits

Swiss-Prot: 96% identical to HLDE_PSEPF: Bifunctional protein HldE (hldE) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03272, D-beta-D-heptose 7-phosphate kinase / D-beta-D-heptose 1-phosphate adenosyltransferase [EC: 2.7.1.- 2.7.7.-] (inferred from 96% identity to pfo:Pfl01_0484)

MetaCyc: 58% identical to fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-4342 [EC: 2.7.7.70]; RXN0-4341 [EC: 2.7.7.70, 2.7.1.167]

Predicted SEED Role

"ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase" in subsystem LOS core oligosaccharide biosynthesis (EC 2.7.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.-.-, 2.7.1.-, 2.7.7.-

Use Curated BLAST to search for 2.7.-.- or 2.7.1.- or 2.7.1.167 or 2.7.7.- or 2.7.7.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WCJ2 at UniProt or InterPro

Protein Sequence (473 amino acids)

>AO353_12970 heptose 1-phosphate adenyltransferase (Pseudomonas fluorescens FW300-N2E3)
MKLSMPRFDQAPVLVVGDVMLDRYWHGGTSRISPEAPVPVVKVEQIEDRPGGAANVALNI
AALGAPASLVGVTGDDEAADSLTNSLKGAGVRALFQRIAHQPTIVKLRVMSRHQQLLRID
FEEPFATDALALGAEVDGLLEGIKVLVLSDYGKGALKNHQVLIQAARARGIPVLADPKGK
DFSIYRGASLITPNLSEFETIVGGCADEHDLVSKGAKLMHDLDLGALLVTRGEHGMTLLR
PDHPALHLPARAREVFDVTGAGDTVISTLAAAIAAGEELPHAVALANLAAGIVVGKLGTA
AISAPELRRAIQREEGSERGVLGLEQLLLAVDDARAHKEKIVFTNGCFDILHAGHVTYLE
QARAQGDRLIVAVNDDASVSRLKGPGRPINSVDRRMAVLAGLGAVDWVISFSEGTPENLL
AQVKPDVLVKGGDYGIDQVVGADIVSAYGGTVKVLGLVENSSTTAIVEKIRKT