Protein Info for AO353_12955 in Pseudomonas fluorescens FW300-N2E3

Annotation: lipid transporter ATP-binding/permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 602 transmembrane" amino acids 26 to 51 (26 residues), see Phobius details amino acids 76 to 99 (24 residues), see Phobius details amino acids 174 to 177 (4 residues), see Phobius details amino acids 180 to 200 (21 residues), see Phobius details amino acids 265 to 288 (24 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 17 to 594 (578 residues), 840 bits, see alignment E=4.5e-257 PF00664: ABC_membrane" amino acids 32 to 307 (276 residues), 172.9 bits, see alignment E=1.2e-54 PF00005: ABC_tran" amino acids 372 to 522 (151 residues), 114.2 bits, see alignment E=7.6e-37

Best Hits

Swiss-Prot: 86% identical to MSBA_PSEPK: Lipid A export ATP-binding/permease protein MsbA (msbA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 87% identity to ppw:PputW619_0531)

MetaCyc: 46% identical to ATP-binding lipopolysaccharide transport protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-236 [EC: 7.5.2.6]; 7.5.2.6 [EC: 7.5.2.6]

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA" in subsystem ZZ gjo need homes

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 7.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H024 at UniProt or InterPro

Protein Sequence (602 amino acids)

>AO353_12955 lipid transporter ATP-binding/permease (Pseudomonas fluorescens FW300-N2E3)
MSAAPPNAEQESSLKIYLRLLGYVKPYVGIFLLSIVGFVIFASTQPMLAGILKYFVDGLS
NPEAVLFPHTPYLRELKLLVAVPLLIILIAAWQGLGSFLSNYYLAKVSLGLVHDLRVELF
NKLLVLPNRYFDTHNSGHLISRITFNVTMVTGAATDAIKVVIREGLTVVFLFGYLLWMNW
KLTLVMLAILPLISFMVGNTSKKFRKQSKKIQVAMGDVTHVASETIQGYRVVRSFGGESY
EQQRFAKASQSNTDKQLRMTRTSAIYTPALQLVIYSAMAALMFLVLFLRGDATAGDLVAY
ITAAGLLPKPIRQLSEVSSTIQKGVAGAESIFEQLDVEPELDAGTIERERISGRLDVHNL
SFTYPGTDREVLKNISFTATPGQMIALVGRSGSGKSTLASLIPRFYHHETGQILLDEVEI
EDYRLRNLRKHVSQVTQHVTLFNDTVANNIAYGDLAGAPRADIEKAAQDAYAMDFISQLP
NGLDTEVGENGVLLSGGQRQRLAIARALLKNAPLLILDEATSALDTESERHIQAALDKVM
KGRTTLVIAHRLSTIEKADLILVMDHGEIVERGTHAQLLAQGGYYSRLHAMGLDEPSPAD
IA