Protein Info for AO353_12845 in Pseudomonas fluorescens FW300-N2E3

Annotation: peptide methionine sulfoxide reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 215 PF01625: PMSR" amino acids 52 to 204 (153 residues), 209.9 bits, see alignment E=1.1e-66 TIGR00401: peptide-methionine (S)-S-oxide reductase" amino acids 52 to 204 (153 residues), 220.1 bits, see alignment E=8.9e-70

Best Hits

Swiss-Prot: 82% identical to MSRA_PSESM: Peptide methionine sulfoxide reductase MsrA (msrA) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K07304, peptide-methionine (S)-S-oxide reductase [EC: 1.8.4.11] (inferred from 91% identity to pfl:PFL_0506)

MetaCyc: 61% identical to methionine sulfoxide reductase A (Escherichia coli K-12 substr. MG1655)
L-methionine (S)-S-oxide reductase. [EC: 1.8.4.13]; Peptide-methionine (S)-S-oxide reductase. [EC: 1.8.4.13, 1.8.4.11]

Predicted SEED Role

"Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11)" (EC 1.8.4.11)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.8.4.11

Use Curated BLAST to search for 1.8.4.11 or 1.8.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VPC1 at UniProt or InterPro

Protein Sequence (215 amino acids)

>AO353_12845 peptide methionine sulfoxide reductase (Pseudomonas fluorescens FW300-N2E3)
MVLRSEILVNKNVLPTKEQALPGRETPIKVPDEHFVNGHPLLGPFPGNVDFAIFGLGCFW
GAERRFWQREGVYSTSVGYAGGFTPNPTYEEVCSGLTGHSEVVLVVYEPEKVSYEQLLAM
FWELHNPTQGMRQGNDIGTQYRSVIYATNPSQLAAAKASKEVFQAELTKAGLGTITTEIE
EAPTFYYAEAYHQQYLAKNPEGYCGIGGTGVTCPI