Protein Info for AO353_12840 in Pseudomonas fluorescens FW300-N2E3

Annotation: lactate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 45% identical to RLMJ_ECOLI: Ribosomal RNA large subunit methyltransferase J (rlmJ) from Escherichia coli (strain K12)

KEGG orthology group: K07115, (no description) (inferred from 98% identity to pfs:PFLU0456)

MetaCyc: 45% identical to 23S rRNA m6A2030 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6998 [EC: 2.1.1.266]

Predicted SEED Role

"Protein involved in catabolism of external DNA"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.266

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WJR9 at UniProt or InterPro

Protein Sequence (278 amino acids)

>AO353_12840 lactate dehydrogenase (Pseudomonas fluorescens FW300-N2E3)
MNYRHAFHAGNHADVFKHLTLTRLIALMSRKEQPFAYLDTHAGIGLYDLQGDQASRTGEY
LEGIARLWGEQDLPPLTADYMRVLHEMNPDGELRYYPGSPELARRLTRPQDRVLLNEKHP
EDGLLLKDNMKGDRRVKVHLGEGWHVPRALLPVPEKRGLMLIDPPFEKLDEMQRCAASLK
EAIGRMRQTVAAIWYPVKDQRMLRRFYQDLAGTGAPKLLRVELLVHPLDTPNSLNGSGLA
IANPPWGLEEELRELLPWLSKKLGQTQGGWQMDWLIAE