Protein Info for AO353_12550 in Pseudomonas fluorescens FW300-N2E3

Annotation: type II secretion system protein GspE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 TIGR02533: type II secretion system protein E" amino acids 9 to 489 (481 residues), 664.4 bits, see alignment E=5e-204 PF00437: T2SSE" amino acids 120 to 386 (267 residues), 358.5 bits, see alignment E=9.4e-112

Best Hits

Swiss-Prot: 59% identical to GSPE1_DICCH: Type II secretion system protein E (outE) from Dickeya chrysanthemi

KEGG orthology group: K02454, general secretion pathway protein E (inferred from 67% identity to pmk:MDS_1732)

Predicted SEED Role

"General secretion pathway protein E"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WCC8 at UniProt or InterPro

Protein Sequence (493 amino acids)

>AO353_12550 type II secretion system protein GspE (Pseudomonas fluorescens FW300-N2E3)
MTSLTGYSEQLPFGFARRLGVLLEGSGEALSLSIRSDTPLTALAEVRRVTGRDLPVQVLE
PAMFATRLASAYGVGQSAAEQVAQGLDEELDLLSLADQLPQTADLLEQDGDAPIIRLINA
LLSEAVREKASDVHLETFEQYLLVRMRVDGQLREVLRPRRELANLLVSRIKVMARLDIAE
KRVPQDGRIALRLAGHEVDVRVSTLPSAHGERVVLRLLDKQAGRLDLQRLGMPPGTLARF
QRILGRPHGIFLVTGPTGSGKTTSLYAALASLNDQTRNILTVEDPIEYHLPGIGQTPVNP
KVEMTFARGLRAILRQDPDVVMVGEIRDRETAEIAVQASLTGHLVLSTLHTNSAVGAVTR
LLDMGVDAYLLASSLIGVLAQRLLRTLCPHCKHAYEADAVDFQSLGIAGDRTVRLFKANG
CARCQQGYRGRVGMYELISMSPTLSALVHQGASEQALADEARTLSHSLFQDGQRLVLEGV
TSLDELLRVSQED