Protein Info for AO353_12530 in Pseudomonas fluorescens FW300-N2E3

Annotation: multidrug transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 489 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF03067: LPMO_10" amino acids 33 to 206 (174 residues), 135 bits, see alignment E=4.9e-43 PF18416: GbpA_2" amino acids 218 to 315 (98 residues), 78.6 bits, see alignment E=3.7e-26

Best Hits

KEGG orthology group: K03933, chitin-binding protein (inferred from 38% identity to she:Shewmr4_3027)

Predicted SEED Role

"Chitin binding protein" in subsystem Chitin and N-acetylglucosamine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H014 at UniProt or InterPro

Protein Sequence (489 amino acids)

>AO353_12530 multidrug transporter (Pseudomonas fluorescens FW300-N2E3)
MKNTIFKGGALTGCASSLTLLAALLSSQHVAAHGYLNEPPSRAFLCQKGLNKDCGGAQYE
PQSVGETFKGFPNGVGGAPGQGPIDGKIASGGIAMFSAVDAQSATRWHLTEIKDRSLDFD
WKYTAAHPATKHEYFITKNGWNPNEPLKRASFDTTPFCFVDGGNVLPVSGDKHACVIPDD
KTGHHVILGIWTVGDTAAAFHNVVDVNILAEAALPGGWNSVGAITPATTLIGGDKVKARA
FTHSGESAQYSVEITVDNADEGKPENWSFKLAEQINKTQTLIKAGIRDEQGNIEPVKGVN
KLYARKESSITRYEVALDMQEDTGARMEFGGLAPEHELVKGVGKVAVPIVNNRTMIIEAT
IFDDSNKQVGFTRQQVAAQSNTVLSVDVRSAPGKHGVKLVGTTEDGRTTRQAFEETQFTG
EGGAQEHDFEFPNNVRQYTAGTKVLQPKNGLVYECKPFPYSGYCMQYSETAQGFEPGYGS
DWTMAWDQK