Protein Info for AO353_12415 in Pseudomonas fluorescens FW300-N2E3

Annotation: poly(R)-hydroxyalkanoic acid synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01839: poly(R)-hydroxyalkanoic acid synthase, class II
  • amino acids 1 to 559 (559 residues), 1045.4 bits, E=0
• PF07167: PhaC_N
  • amino acids 75 to 243 (169 residues), 218.7 bits, E=4.3e-69
• PF00561: Abhydrolase_1
  • amino acids 239 to 486 (248 residues), 102.8 bits, E=2.5e-33

Best Hits

Swiss-Prot: 85% identical to PHAC1_PSEOL: Poly(3-hydroxyalkanoate) polymerase 1 (phaC1) from Pseudomonas oleovorans

KEGG orthology group: K03821, polyhydroxyalkanoate synthase [EC: 2.3.1.-] (inferred from 93% identity to pba:PSEBR_a410)

MetaCyc: 84% identical to poly[R)-3-hydroxydecanoate polymerase (Pseudomonas putida GPo1)
RXN-11796 [EC: 2.3.1.304]

Predicted SEED Role

"Polyhydroxyalkanoic acid synthase" in subsystem Polyhydroxybutyrate metabolism

MetaCyc Pathways

• polyhydroxybutanoate biosynthesis (3/3 steps found)
• polyhydroxydecanoate biosynthesis (2/3 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.- or 2.3.1.304

Sequence Analysis Tools

See A0A0N9VUA1 at UniProt or InterPro

Protein Sequence (559 amino acids)

>AO353_12415 poly(R)-hydroxyalkanoic acid synthase (Pseudomonas fluorescens FW300-N2E3)
MSNKNNDDLKLQASENTLGLNPVIGLRRKDLLTSARMVLTQAIKQPIHSVKHVAHFGIEL
KNVMLGRSELQPESDDRRFNDPAWSQNPLYRRYLQTYLAWRKELHDWIGESNLSTQDISR
AHFVINLMTEAMAPTNSAANPAAVKRFFETGGKSLLDGLSHLAKDLVHNGGMPSQVNMGA
FEVGKSLGTTEGAVVFRNDVLELIQYRPITEQVHERPLLVVPPQINKFYVFDLSPDKSLA
RFCLRNNVQTFIVSWRNPTKAQREWGLSTYIEALKEAVDVVTAITGSKDVNMLGACSGGI
TCTALLGHYAAIGEKKVNALTLLVSVLDTTLDSQVALFVDEQTLEAAKRHSYQAGVLEGR
DMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNNDTTRLPAAFHGDLIELFKNNP
LIRPDALEVCGTPIDLKQVTADVYSLAGTNDHITPWKSCYKSAQLFGGKVEFVLSSSGHI
QSILNPPGNPKSRYMTSTDMPVHANDWLENSTKHTDSWWLHWQAWQAERSGKLKKAPTVL
GNKTYAAGEASPGTYVHER