Protein Info for AO353_12285 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): N-formylglutamate deformylase (EC 3.5.1.68)
Rationale: Specifically important for: L-Histidine. KEGG suggests formimidoyl-L-glutamate as the substrate, while SEED suggests N-formylglutamate. This gene is cofit with iminohydrolase (which produces N-formylglutamate) which suggests that the SEED annotation is correct. (SEED_correct)
Original annotation: N-formylglutamate deformylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02017: N-formylglutamate deformylase
  • amino acids 4 to 262 (259 residues), 395.5 bits, E=5.7e-123
• PF05013: FGase
  • amino acids 13 to 230 (218 residues), 251.5 bits, E=5.2e-79

Best Hits

KEGG orthology group: K01479, formiminoglutamase [EC: 3.5.3.8] (inferred from 90% identity to pfo:Pfl01_0368)

MetaCyc: 76% identical to N-formylglutamate amidohydrolase (Pseudomonas putida)
N-formylglutamate deformylase. [EC: 3.5.1.68]

Predicted SEED Role

"N-formylglutamate deformylase (EC 3.5.1.68)" in subsystem Histidine Degradation (EC 3.5.1.68)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation I (4/4 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.68 or 3.5.3.8

Sequence Analysis Tools

See A0A0N9WHL9 at UniProt or InterPro

Protein Sequence (266 amino acids)

>AO353_12285 N-formylglutamate deformylase (EC 3.5.1.68) (Pseudomonas fluorescens FW300-N2E3)
VDKVLNFKQGRVPLLISMPHAGVRLTPAVEAGLIPEAKSLPDTDWHIPTLYEFAAELGAS
TLAAEYSRFVIDLNRPSDDKPMYVGATTGLYPATLFDGVPLFREGLEPSAEERASYLEKV
WTPYHSTLQQELARLKAEFGYALLFDAHSIRSVIPHLFDGKLPDFNLGTFNGASCDPQLA
SQLEAICARHTDYSHVLNGRFKGGHITRHYGNPAENIHAVQLELGQCTYMEEVEPFRYRP
DLAAPTQVVLKELLQGLLAWGQKHYA