Protein Info for AO353_12275 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): histidine permease
Rationale: Specific phenotype on histidine. 86% identical to PFLU0368, which is required for histidine utilization (PMCID: PMC1950622). SEED_correct
Original annotation: proline-specific permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 transmembrane" amino acids 19 to 38 (20 residues), see Phobius details amino acids 44 to 63 (20 residues), see Phobius details amino acids 89 to 119 (31 residues), see Phobius details amino acids 125 to 143 (19 residues), see Phobius details amino acids 155 to 179 (25 residues), see Phobius details amino acids 200 to 223 (24 residues), see Phobius details amino acids 243 to 262 (20 residues), see Phobius details amino acids 281 to 301 (21 residues), see Phobius details amino acids 332 to 353 (22 residues), see Phobius details amino acids 359 to 381 (23 residues), see Phobius details amino acids 402 to 424 (23 residues), see Phobius details amino acids 430 to 448 (19 residues), see Phobius details PF00324: AA_permease" amino acids 16 to 450 (435 residues), 383.7 bits, see alignment E=1.2e-118 PF13520: AA_permease_2" amino acids 19 to 442 (424 residues), 121 bits, see alignment E=6.5e-39

Best Hits

Swiss-Prot: 63% identical to PROY_SALTY: Proline-specific permease ProY (proY) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 86% identity to pfs:PFLU0368)

MetaCyc: 47% identical to aromatic amino acid:H+ symporter AroP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-56; TRANS-RXN-76; TRANS-RXN-77

Predicted SEED Role

"Histidine transport protein (permease)" in subsystem Histidine Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VTP5 at UniProt or InterPro

Protein Sequence (468 amino acids)

>AO353_12275 histidine permease (Pseudomonas fluorescens FW300-N2E3)
MQKPANGLKRGLSARHIRFMALGSAIGTGLFYGSASAIQMAGPAVLLAYLIGGAAVFMVM
RALGEMAVHNPVAGSFGQYASTYLGPMAGFILGWTYAFEMVIVGMADVTAFGIYMGFWFP
EVSRWIWVLGVVSIVGGLNLCNVKVFGEMEFWLSLLKVAAIVAMILGGFGIMLFGISTAP
GQVTDISNLWTQGGFMPNGMGGLIASFAVVMFAFGGIEIIGVTAGEAKDPQHVLPRAINA
VPLRILLFYVLTMLVLMSIFPWQQIGSQGSPFVQIFDKLGISSAATILNIVVITAAISAI
NSDIFGAGRMMFGLAQQGHAPKGFAHLSRNGVPWMTVVVMSVALLLGVLLNYLIPENVFL
LIASIATFATVWVWLMILFTQVAMRRSMTAEQVAQLKFPVPFWPYAPMAAIAFMLFVFGV
LGYFPDTQAALIVGVVWIVLLVLAYLMWVKPAAGQAALVARDPSFSNR