Protein Info for AO353_12250 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Urocanate hydratase (EC 4.2.1.49)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (UROCANATE-HYDRATASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 TIGR01228: urocanate hydratase" amino acids 20 to 563 (544 residues), 1018.9 bits, see alignment E=0 PF17391: Urocanase_N" amino acids 21 to 147 (127 residues), 208.7 bits, see alignment E=3e-66 PF01175: Urocanase" amino acids 150 to 358 (209 residues), 298.9 bits, see alignment E=3.1e-93 PF17392: Urocanase_C" amino acids 361 to 555 (195 residues), 306.9 bits, see alignment E=7.6e-96

Best Hits

Swiss-Prot: 96% identical to HUTU_PSEFS: Urocanate hydratase (hutU) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 95% identity to pba:PSEBR_a378)

MetaCyc: 92% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H007 at UniProt or InterPro

Protein Sequence (567 amino acids)

>AO353_12250 Urocanate hydratase (EC 4.2.1.49) (Pseudomonas fluorescens FW300-N2E3)
VTDNTQKPTPVTFTKHRDVEIRAPRGNKLTAKSWLTEAPLRMLMNNLDPEVAENPKELVV
YGGIGRAARNWECYDKIVESLTNLNDDETLLVQSGKPVGVFKTHSNAPRVLIANSNLVPH
WASWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFVEAGRQHYNDNLTGRWVL
TAGLGGMGGAQPLAATLAGACSLNIECQQVSIDFRLKSRYVDEQAKDLDDALARIAKYTK
EGKAISIALLGNAAEILPELVRRGVRPDMVTDQTSAHDPLNGYLPAGWTWEEYRARAKTE
PAAVIKAAKQSMAVHVKAMLDFQKQGIPTFDYGNNIRQMAQEEGVENAFDFPGFVPAYIR
PLFCRGIGPFRWAALSGNAEDIYKTDAKVKELIPDDAHLHNWLDMARERISFQGLPARIC
WVGLGLRAKLGLAFNEMVRSGELSAPIVIGRDHLDSGSVASPNRETESMQDGSDAVSDWP
LLNALLNTASGATWVSLHHGGGVGMGFSQHSGMVIVCDGTDEAAERIARVLHNDPATGVM
RHADAGYQIAIDCAKEQGLNLPMITGK