Protein Info for AO353_12150 in Pseudomonas fluorescens FW300-N2E3

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 PF00989: PAS" amino acids 9 to 105 (97 residues), 29.8 bits, see alignment E=1.1e-10 PF08448: PAS_4" amino acids 12 to 109 (98 residues), 25.8 bits, see alignment E=2.1e-09 PF00512: HisKA" amino acids 136 to 189 (54 residues), 57.3 bits, see alignment 2.6e-19 PF02518: HATPase_c" amino acids 237 to 355 (119 residues), 61.1 bits, see alignment E=2.6e-20

Best Hits

KEGG orthology group: K07708, two-component system, NtrC family, nitrogen regulation sensor histidine kinase GlnL [EC: 2.7.13.3] (inferred from 97% identity to pfo:Pfl01_0340)

Predicted SEED Role

"Nitrogen regulation protein NtrB (EC 2.7.13.3)" (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VTL7 at UniProt or InterPro

Protein Sequence (361 amino acids)

>AO353_12150 histidine kinase (Pseudomonas fluorescens FW300-N2E3)
MTISDALHRLLLDNLTTATILLNADLRLEYMNPAAEMLLAISGQRSHGQFISELFTESTE
ALNSLRQAVEQAHPFTKREAMLTALTGQTLTVDYAVTPILSNGDTLLLLEVHPRDRLLRI
TKEEAQLSKQETSKMLVRGLAHEIKNPLGGIRGAAQLLARELPEESLKDYTNVIIEEADR
LRNLVDRMLGSNKLPSLALCNVHEVLERVCHLVEAESQGCITLVRDYDPSIPDVLIDREQ
MIQAVLNIVRNAMQAISTQNELRLGRITLRSRAMRQFTIGHVRHRLVTKLEIIDNGPGIP
ADLQETIFFPMVSGRPDGTGLGLAITQNIISQHQGLIECDSHPGHTTFSIFLPLEQGATS
T