Protein Info for AO353_12010 in Pseudomonas fluorescens FW300-N2E3

Annotation: plasmid maintenance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 135 PF01850: PIN" amino acids 4 to 125 (122 residues), 72.9 bits, see alignment E=1.8e-24

Best Hits

Swiss-Prot: 71% identical to VAPC_SALTY: tRNA(fMet)-specific endonuclease VapC (vapC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K07062, (no description) (inferred from 81% identity to pst:PSPTO_1045)

Predicted SEED Role

"VapC toxin protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WC58 at UniProt or InterPro

Protein Sequence (135 amino acids)

>AO353_12010 plasmid maintenance protein (Pseudomonas fluorescens FW300-N2E3)
MIKYMLDTNICIFTIKNKPQVVREAFNRHHGQLCISAVTLMELIYGAEKSAAPEKNLAVI
EGFVARLEVLPFDYEAAAHTGMIRSELAKAGTPIGPYDHMIAGHARSQGFIVVTNNLREF
ERVPGLRVEDWVHLD