Protein Info for AO353_11910 in Pseudomonas fluorescens FW300-N2E3

Annotation: N-carbamoylputrescine amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03381: N-carbamoylputrescine amidase
  • amino acids 5 to 282 (278 residues), 487.8 bits, E=4.6e-151
• PF00795: CN_hydrolase
  • amino acids 6 to 266 (261 residues), 188.4 bits, E=7.6e-60

Best Hits

Swiss-Prot: 64% identical to AGUB_ORYSJ: N-carbamoylputrescine amidase (CPA) from Oryza sativa subsp. japonica

KEGG orthology group: K12251, N-carbamoylputrescine amidase [EC: 3.5.1.53] (inferred from 94% identity to pfl:PFL_0307)

MetaCyc: 86% identical to N-carbamoylputrascine amidohydrolase subunit (Pseudomonas aeruginosa)
N-carbamoylputrescine amidase. [EC: 3.5.1.53]

Predicted SEED Role

"N-carbamoylputrescine amidase (3.5.1.53)" in subsystem Arginine and Ornithine Degradation or Polyamine Metabolism

MetaCyc Pathways

• L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) (3/3 steps found)
• putrescine biosynthesis II (3/3 steps found)
• superpathway of polyamine biosynthesis II (6/8 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.53

Use Curated BLAST to search for 3.5.1.53

Sequence Analysis Tools

See A0A0N9VTI5 at UniProt or InterPro

Protein Sequence (292 amino acids)

>AO353_11910 N-carbamoylputrescine amidase (Pseudomonas fluorescens FW300-N2E3)
MSRIVTVAATQMACSWDLEANIETAEKLVREAAAKGAQIILIQELFETPYFCQKPNPDYL
QLATSVEDNVAIKHFQKVAKELQVVLPISFFELAGRARFNSIAIIDADGSNLGIYRKSHI
PDGPGYHEKYYFNPGDTGFKVWNTRYAKIGVGICWDQWFPECARSMALQGAEILFYPTAI
GSEPHDKSISSRDHWQRVQQGHAGANLMPLIASNRIGNEEQDGYDITFYGSSFIANQFGE
KVQELNETEEGVLVQSFDLDELEHIRSAWGSFRDRRPNLYGAIKTLDGSLES