Protein Info for AO353_11520 in Pseudomonas fluorescens FW300-N2E3

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 TIGR00229: PAS domain S-box protein" amino acids 36 to 164 (129 residues), 45.2 bits, see alignment E=9.8e-16 PF08447: PAS_3" amino acids 63 to 151 (89 residues), 71.4 bits, see alignment E=9.6e-24 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 164 to 326 (163 residues), 151.6 bits, see alignment E=1.7e-48 PF00990: GGDEF" amino acids 168 to 325 (158 residues), 151.7 bits, see alignment E=2.3e-48

Best Hits

KEGG orthology group: None (inferred from 87% identity to pfo:Pfl01_0190)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VU27 at UniProt or InterPro

Protein Sequence (329 amino acids)

>AO353_11520 diguanylate cyclase (Pseudomonas fluorescens FW300-N2E3)
MVNKNLEDLPYPQWPEAAQTLMALMHAQGEVARLSEREQLFSSLLVSVNAVLWAFNWETR
QVLYVSPAYERIFGRSAGLLLADYHQWRDSVYPDDLDYAERSLAQVLEKGAVEDREYRIF
AADGQVRWLSDKCFINRQAEPGQPLIVVGFAEDITEKKQLETELQRLATTDVLTQSSNRR
HFFECASHEFEQARLQGSPLAFLLLDIDDFKLINDTYGHQQGDQVLQQLAECGRAALRRG
DLFGRIGGEEFAAVFPGCAPEMARQVAERLQREIQRLSFSHDGQTFSITVSQGLTSLTAE
DAAIDSLYARADAAMYRAKRQGKNRIISG