Protein Info for AO353_11510 in Pseudomonas fluorescens FW300-N2E3
Updated annotation (from data): 5-aminovalerate transaminase (EC 2.6.1.48)
Rationale: Specifically important for utilizing L-Lysine. This confirms that it is involved in L-lysine catabolism via 5-aminovalerate. A milder phenotype on putrescine suggests that it is also likely to be a 4-aminobutyrate transaminase.
Original annotation: 4-aminobutyrate aminotransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 87% identical to DAVT_PSEPK: 5-aminovalerate aminotransferase DavT (davT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K14268, 5-aminovalerate aminotransferase [EC: 2.6.1.48] (inferred from 97% identity to pfl:PFL_0186)MetaCyc: 97% identical to 4-aminobutyrate transaminase subunit (Pseudomonas fluorescens)
4-aminobutyrate transaminase. [EC: 2.6.1.19]
Predicted SEED Role
"5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" in subsystem Lysine degradation (EC 2.6.1.19, EC 2.6.1.48)
MetaCyc Pathways
- L-lysine degradation X (6/6 steps found)
- superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
- L-lysine degradation IV (5/5 steps found)
- superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
- 4-aminobutanoate degradation II (2/2 steps found)
- 4-aminobutanoate degradation III (2/2 steps found)
- L-lysine degradation I (5/7 steps found)
- superpathway of 4-aminobutanoate degradation (2/3 steps found)
- 4-aminobutanoate degradation I (1/2 steps found)
- GABA shunt II (2/4 steps found)
- GABA shunt I (1/4 steps found)
- L-lysine degradation III (2/6 steps found)
- 4-aminobutanoate degradation V (2/7 steps found)
- nicotine degradation I (pyridine pathway) (6/17 steps found)
- superpathway of L-lysine degradation (18/43 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Butanoate metabolism
- Glutamate metabolism
- Lysine degradation
- Propanoate metabolism
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.19
Use Curated BLAST to search for 2.6.1.19 or 2.6.1.48
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9WHC0 at UniProt or InterPro
Protein Sequence (425 amino acids)
>AO353_11510 5-aminovalerate transaminase (EC 2.6.1.48) (Pseudomonas fluorescens FW300-N2E3) MSKTNASLMKRREAAVPRGVGQIHPIFADHAKNSTVTDVEGREFIDFAGGIAVLNTGHLH PKVIAAVTEQLNKLTHTCFQVLAYEPYVELCEKINAKVPGDFAKKTLLVTTGSEAVENSI KIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALYPNELHGVS IDDSIASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVAPKEFMKRLRALCDQHGILLIAD EVQTGAGRTGTFFAMEQMGVAADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYA GSPIACAAALAVMEVFEEEHLLDRCKAVGERLVTGLKAIQAKYPVIGEVRALGAMIAVEL FENGDSHKPNAAAVAKVVAKARDKGLILLSCGTYGNVLRVLVPLTAPDEQLDKGLAIMEE CFSEL