Protein Info for AO353_10970 in Pseudomonas fluorescens FW300-N2E3

Annotation: diguanylate phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 537 signal peptide" amino acids 1 to 39 (39 residues), see Phobius details transmembrane" amino acids 248 to 270 (23 residues), see Phobius details PF12792: CSS-motif" amino acids 47 to 242 (196 residues), 91.6 bits, see alignment E=5e-30 PF00563: EAL" amino acids 280 to 511 (232 residues), 216.6 bits, see alignment E=3.3e-68

Best Hits

KEGG orthology group: None (inferred from 83% identity to pfo:Pfl01_0048)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WNM4 at UniProt or InterPro

Protein Sequence (537 amino acids)

>AO353_10970 diguanylate phosphodiesterase (Pseudomonas fluorescens FW300-N2E3)
MAVAGETLRSWFYRPWSLAILAAALSAALLLAGSLGVAQRQVQQRESEQMNAQGERFLER
LEQLFGQLREGLDDLEAQPLRNCDADMIATLQQVSFNYRFVYEAAYIDSTQFCSSRPRQD
GLSAIRPPDIKGPTYSYWLNTSTEPDEDRAALMLGRGNFRVATSRGHLTDMVDLTPGSSL
LVVVDHGARAIPVLGTPQAWPLSDPWSSFSSEPLQVTKTRLIYRMPTNNPEYQLVLISPR
TASHLPTLWWWLVPASLVLGLSIGGLVFLMARQRQSLGGELQGALKRGELQVLYQPIFDL
KSRHCVGAEALLRWRRPDGTLTSPDLFIPMAEDSGQIRQITDFVLQRLLEQLGKLLRANP
QLYISVNLAACDVMVPRVGQVMAGLLATHRVAARQIAFEVTERGLIDVVVARENLQALRD
AGHQVLIDDFGTGYCSLAYLQTLPVDYLKIDKAFIDALGHDAASSGVAPHIIRMAHALQL
KVIAEGIEHEAQATLLSSEGVNYGQGWLFAHALTATQFIELITRGRRLASRRLDDEA