Protein Info for AO353_10930 in Pseudomonas fluorescens FW300-N2E3

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 923 TIGR00593: DNA polymerase I" amino acids 6 to 923 (918 residues), 940.5 bits, see alignment E=6.3e-287 PF02739: 5_3_exonuc_N" amino acids 6 to 167 (162 residues), 180.3 bits, see alignment E=4.8e-57 PF01367: 5_3_exonuc" amino acids 168 to 270 (103 residues), 103.5 bits, see alignment E=1.5e-33 PF01612: DNA_pol_A_exo1" amino acids 316 to 510 (195 residues), 124.8 bits, see alignment E=6.9e-40 PF00476: DNA_pol_A" amino acids 548 to 921 (374 residues), 540.7 bits, see alignment E=3.8e-166

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 86% identity to pfo:Pfl01_0056)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZX6 at UniProt or InterPro

Protein Sequence (923 amino acids)

>AO353_10930 DNA polymerase I (Pseudomonas fluorescens FW300-N2E3)
MSQAPLVLVDGSSYLYRAFHALPPLTTSKGLPTGAVKGVLNMLKSLRKQYPDSPFAVVFD
AKGGTFRDDLYAEYKANRPSMPDDMRVQIEPLHASVIALGFPLLCVDGVEADDVIGTLAR
SSAAADRPVVISTGDKDMAQLVDGHITLVNTMTGSAMDVAGVKEKFGVAPEQIIDYLALM
GDSSDNIPGVPGIGPKTASGLLVGVNGGLTELYAQLDIVPTLPIRGAKTLPAKLEEHKEM
AFLSYQLATIKVDVPLDVGLDDLHLREPDREKLAELYTLLEFKSWFEEVQRDAKRAGQEV
VAAVEEAVADSDGPHYTTVLTQADFDVWLKKLQNATLFAFDTETTGIDAQQAQLVGLSFA
VQANEAAYIPLTHSYMGVPDQLDRDTVLRALKPILEDPSKLKVGQHAKFDMNILANCAIG
GDQSCGIMVQGIAFDTMLESYVLDSTATRHDMDSLALKYLGYTTTSFQDIAGKGVKQLTF
DQISLELAGPYAAEDADVTLRLHQTLHEKLDAIPSLSKVLSEIEMPLVPVLARIERQGAL
VDANLLGIQSVELGDKLVALEREAFAIAGEEFNLGSPKQLGVILYEKLGLPILSKTAKGQ
ASTAEAVLAELAEQDYPLPKVLMQYRSLSKLKSTYTDRLPEQINPRTGRIHTSYHQAVAA
TGRLSSSDPNLQNIPIRTAEGRRIRQAFIAPKGYKLLAADYSQIELRIMAHLAKDEGLLH
AFRNDLDVHKATAAEVFGVELADVTTDQRRSAKAINFGLIYGMSAFGLAKQIGVDRKQSQ
AYIDRYFARYPGVLEYMERTRAQAAEQGFVETIFGRRLYLPDINAKNPALRKGAERTAIN
APMQGTAADIIKKAMVAVDNWLLTSGLDAKVILQVHDELVLEVREDLIDQVREEIRVHMS
SAATLDVPLLVEVGVGNNWDEAH