Protein Info for AO353_10815 in Pseudomonas fluorescens FW300-N2E3

Annotation: carbonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00484: Pro_CA
  • amino acids 59 to 214 (156 residues), 175.7 bits, E=4.2e-56

Best Hits

Swiss-Prot: 51% identical to CYNT_PSEAE: Carbonic anhydrase (cynT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01673, carbonic anhydrase [EC: 4.2.1.1] (inferred from 91% identity to pba:PSEBR_a98)

Predicted SEED Role

"Carbonic anhydrase (EC 4.2.1.1)" in subsystem Carboxysome or Cyanate hydrolysis (EC 4.2.1.1)

MetaCyc Pathways

• cyanate degradation (3/3 steps found)
• CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (6/11 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• 3-hydroxypropanoate cycle (5/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• glyoxylate assimilation (4/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (5/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.1

Use Curated BLAST to search for 4.2.1.1

Sequence Analysis Tools

See A0A0N9WF48 at UniProt or InterPro

Protein Sequence (243 amino acids)

>AO353_10815 carbonate dehydratase (Pseudomonas fluorescens FW300-N2E3)
MSDKDKQPLAASASALPEAESADAALQHIVDGFLHFHHEVFPQQEELFKKLATAQRPRAM
FIACADSRIVPELITQSAPGDLFVTRNVGNVVPPYGQMNGGVSTAIEYAVLALGVQHIII
CGHSDCGAMRAVLNPQSLEKMPTVKAWLRHAEVAKTMVQENCHCADENESMQVLTEENVI
AQLQHLRTHPSVASRMANGQLFIHGWVYSIETSEIKAYDADKGCFLPLDGSHPIPVATPK
ARF