Protein Info for AO353_10800 in Pseudomonas fluorescens FW300-N2E3

Annotation: alcohol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF13442: Cytochrome_CBB3" amino acids 28 to 123 (96 residues), 23.3 bits, see alignment E=6.6e-09 amino acids 313 to 396 (84 residues), 38.2 bits, see alignment E=1.5e-13 PF00034: Cytochrom_C" amino acids 29 to 125 (97 residues), 25.7 bits, see alignment E=2.4e-09 amino acids 185 to 286 (102 residues), 21.9 bits, see alignment E=3.7e-08 amino acids 313 to 400 (88 residues), 53.9 bits, see alignment E=3.8e-18

Best Hits

Swiss-Prot: 66% identical to GADH2_PANCY: Gluconate 2-dehydrogenase cytochrome c subunit from Pantoea cypripedii

KEGG orthology group: None (inferred from 84% identity to pfl:PFL_0055)

MetaCyc: 84% identical to D-gluconate dehydrogenase cytochrome c subunit (Pseudomonas fluorescens)
Gluconate 2-dehydrogenase (acceptor). [EC: 1.1.99.3]

Predicted SEED Role

"Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, cytochrome c" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.3

Use Curated BLAST to search for 1.1.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WBN3 at UniProt or InterPro

Protein Sequence (435 amino acids)

>AO353_10800 alcohol dehydrogenase (Pseudomonas fluorescens FW300-N2E3)
MKNLVIATLALLGSCSISAAEDASQALIKQGEYLARAGDCVACHTAKGGKPFAGGLPMET
PIGTIYSTNITPDKTGLGDYSFDDFDKAVRHGVAKNGSTLYPAMPYPSYARVSDADMKAL
YAYFMKGVAPVTQENKGSDIPWPLSMRWPLTGWRWMFAPSVVAYQPADGKEAAISRGAYL
VEGLGHCGACHTPRALTMQEKALSANEGSAFLSGSAPLEGWIAKSLRGDHKDGLGSWSEE
QLVQFLKTGRSDRSAVFGGMSDVVEHSMQYMSDDDLTAIARYLKSLPANDPNDQPHPYDP
QVAQALWKGDDSKPGASVYIDNCAACHRTDGHGYTRVFPALAGNPVLQSDDPVSLINIVL
KGATLPATHTAPSTFTMPAFAWRLSDQEVADVVNFIRTSWGNKGAQIKPADVADVRKDEV
KSMPADKSGKLTSSN