Protein Info for AO353_10680 in Pseudomonas fluorescens FW300-N2E3

Annotation: glycine/betaine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR03414: choline ABC transporter, periplasmic binding protein" amino acids 22 to 306 (285 residues), 437.7 bits, see alignment E=9.9e-136 PF04069: OpuAC" amino acids 27 to 277 (251 residues), 215.6 bits, see alignment E=4.8e-68

Best Hits

KEGG orthology group: K02002, glycine betaine/proline transport system substrate-binding protein (inferred from 94% identity to pba:PSEBR_a66)

Predicted SEED Role

"L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (TC 3.A.1.12.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZX0 at UniProt or InterPro

Protein Sequence (306 amino acids)

>AO353_10680 glycine/betaine ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N2E3)
MQKLSTVLTAGLLALSSVSAYAEQSCDTVKMADPGWSDIAATNAITGFLLEGMGYKAKVD
TLAVPITFGGLKDGQVDVFLGNWMPAQQGFYDKFVANGDVTQLSKNLDGTEFTLAVPDYV
WDAGVHNFADLNKYADKFDKKIYGIGSGAPANISLQEIIKKNDFDLGQWKLIESSEQAML
AEVSRAVKKQKFVTFLGWTPHPMNVQLKMHYLKGGEKYFGDTGSVHTLTRKGYAQACPNV
GKLLTNLSFTQEMENSIMAEVVNKKLSNADAAKAWIKANPAVLDKWLDGVKTVDGKDALP
AVKAKL