Protein Info for AO353_10630 in Pseudomonas fluorescens FW300-N2E3
Annotation: 16S rRNA methyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 87% identical to RSMB_PSESM: Ribosomal RNA small subunit methyltransferase B (rsmB) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
KEGG orthology group: K03500, ribosomal RNA small subunit methyltransferase B [EC: 2.1.1.-] (inferred from 95% identity to pba:PSEBR_a76)MetaCyc: 50% identical to 16S rRNA m5C967 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11591 [EC: 2.1.1.176]
Predicted SEED Role
"Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase (EC 2.1.1.-)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Anthocyanin biosynthesis
- Benzoxazinone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
- Carotenoid biosynthesis - General
- Flavonoid biosynthesis
- Histidine metabolism
- Insect hormone biosynthesis
- Naphthalene and anthracene degradation
- Phenylpropanoid biosynthesis
- Porphyrin and chlorophyll metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.-
Use Curated BLAST to search for 2.1.1.- or 2.1.1.176
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9W526 at UniProt or InterPro
Protein Sequence (436 amino acids)
>AO353_10630 16S rRNA methyltransferase (Pseudomonas fluorescens FW300-N2E3) MNPRLAAAKALAAVLNGKASLNSSLPTQLDKVEDRDRGFTQDLAFGTARWQPRLSALAAK LLQKPFKAADADVEALLLVGLYQLLYTRVPAHAAIGETVGCADKLKKPWAKALLNAVLRR AQRESEALLAELEHDPVVRTAHPRWLQKSLKAFWPEQWEAICAANNAHPPMILRVNRRHH TRDAYLALLSDAGVAAKPCVFSRDGIVLDTPGDVRQLPGFADGWISVQDEAAQLAADLLD LAPGQRVLDACCAPGGKTCHILEVEPKLAGVVAVDLEAKRLVRVRENLERLGLSAELIAA DGRDTQAWWDGKPFQRILLDAPCSATGVIRRHPDIKLTRQPDDIAALAVLQGELLDAMWP TLDVGGILLYATCSTLPTENTEVIEAFLARTPGARELDIASQAGIKQPHGRQLLAQEGGH DGFYYAKLIKIAAARG