Protein Info for AO353_10595 in Pseudomonas fluorescens FW300-N2E3

Annotation: glycine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 684 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 683 (683 residues), 798.9 bits, see alignment E=2.9e-244 PF02092: tRNA_synt_2f" amino acids 6 to 543 (538 residues), 684.3 bits, see alignment E=1.5e-209 PF05746: DALR_1" amino acids 579 to 670 (92 residues), 34.1 bits, see alignment E=2.6e-12

Best Hits

Swiss-Prot: 94% identical to SYGB_PSEF5: Glycine--tRNA ligase beta subunit (glyS) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 94% identity to pfl:PFL_0013)

MetaCyc: 51% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZW8 at UniProt or InterPro

Protein Sequence (684 amino acids)

>AO353_10595 glycine--tRNA ligase subunit beta (Pseudomonas fluorescens FW300-N2E3)
MSAQDFLVELGTEELPPKALNTLADAFLAGIEKGLLAAGLGFEAKQVYAAPRRLAVLITK
LATQQPDRSINLDGPPRQAAFDAEGNPTQAALGFAKKCGVELSEIDQSGPKLRYSQSIAG
KPTVSLLPTIVEDSLNDLPIPKRMRWGARKEEFVRPTQWLVMLLGDQVIDCTILAQKAGR
DSRGHRFHHPESVRITSPANYLNDLRAAYVLADANERRELISTRTAELATRQEGTAIVPP
DLLDEVTALVEWPVPLVCSFEERFLDVPQEALITTMQDNQKYFCLLDADGKLLPRFITVA
NIESKDPQQIIDGNEKVVRPRLTDAEFFFKQDKKQKLEDFNLRLQNVVFQEKLGSVYDKA
ERVSKLAAFIAQRIGGNASWAARAGLLSKCDLATEMVGEFPEMQGVAGYYYALNDGEPED
VALALNEQYMPRGAGAELPTTLTGAAVAIADKLDTLVGIFGVGMLPTGSKDPYALRRAAL
GVLRILIDKKLDLDLTDAVAFAVNAFGSKVKAAGLADSVLEFVFDRLRARYEDEGVDVAT
YLSVRALKPGSALDFDQRVQAVEAFRKLPEAAALAAVNKRVSNLLSKIEGSVPSTVEAKY
FDNAAEFSLYSAIQQADQAVQPMAAARQYSESLARLAALREPVDAFFEAVMVNAEDAKVR
ANRYALLARLRGLFLGVADISLLG