Protein Info for AO353_10380 in Pseudomonas fluorescens FW300-N2E3

Annotation: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 1 to 436 (436 residues), 610.7 bits, E=8.4e-188
• PF12804: NTP_transf_3
  • amino acids 3 to 103 (101 residues), 49.3 bits, E=1.3e-16
• PF00483: NTP_transferase
  • amino acids 6 to 181 (176 residues), 34.6 bits, E=3.1e-12
• PF00132: Hexapep
  • amino acids 248 to 281 (34 residues), 31.6 bits, 1.9e-11
• PF14602: Hexapep_2
  • amino acids 266 to 298 (33 residues), 16.5 bits, see alignment (E = 1.1e-06)
  • amino acids 377 to 410 (34 residues), 21 bits, 4.6e-08

Best Hits

Swiss-Prot: 91% identical to GLMU_PSEPF: Bifunctional protein GlmU (glmU) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 91% identity to pfo:Pfl01_5728)

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• peptidoglycan recycling I (11/14 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (8/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See A0A0N9WBH1 at UniProt or InterPro

Protein Sequence (440 amino acids)

>AO353_10380 bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase (Pseudomonas fluorescens FW300-N2E3)
MRSALPKVLHPVAGNSMLGHVIHSARQLDPQRIHVVIGHGADVVRERLAADDLNFVLQDQ
QLGTGHAVAQAVPFITADTVLILYGDVPLIEVPTLQRLLKLVAPLQMGLLTVELDDPTGY
GRIVRNADGRVTAIVEHKDASEAERMITEGNTGIMSVPASHLGDWMSRLSNNNVQGEYYL
TDVIAMAVSDGLVVDTEQPHDAMEVQGANDRKQLAELERHYQLREGRRLMAQGVTLRDPA
RFDVRGEVTVGRDVLIDINVILEGRVVIEDDVVIGPNCVIKDSTLRKGVVIKANSHIEGA
VLGEGSDAGPFARLRPGTVLEARAHVGNFVELKNAHLGEGAKAGHLTYLGDAEVGARTNI
GAGTITCNYDGVNKWKTVLGEDVFIGSNNSLVAPVDILDGATTAAGSTITSTVDKSQLAV
GRARQKNIDGWKRPVKVQKS