Protein Info for AO353_10340 in Pseudomonas fluorescens FW300-N2E3

Annotation: fatty acid hydroxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 signal peptide" amino acids 5 to 5 (1 residues), see Phobius details transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 44 to 64 (21 residues), see Phobius details amino acids 74 to 93 (20 residues), see Phobius details amino acids 132 to 156 (25 residues), see Phobius details amino acids 264 to 282 (19 residues), see Phobius details amino acids 293 to 313 (21 residues), see Phobius details amino acids 320 to 338 (19 residues), see Phobius details amino acids 344 to 363 (20 residues), see Phobius details PF04116: FA_hydroxylase" amino acids 78 to 211 (134 residues), 95.4 bits, see alignment E=2e-31

Best Hits

KEGG orthology group: None (inferred from 72% identity to spe:Spro_2864)

Predicted SEED Role

"C-5 sterol desaturase (EC 1.3.-.-)" (EC 1.3.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.-.-

Use Curated BLAST to search for 1.3.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VT15 at UniProt or InterPro

Protein Sequence (379 amino acids)

>AO353_10340 fatty acid hydroxylase (Pseudomonas fluorescens FW300-N2E3)
MIDLAFPILFMLLVVIGEMLVVQWRQRDVVNWHDVMFNLNSGHLMLWLFRGLEITCYGYV
ASHFSLSLFESWPPVLTWVFALFAWDFGFYWLHRAHHYLRVLWAVHVVHHQGEHFNLSLG
VRNSWYSSLTSIPFFMLLAVLGVPLSVFVTVSILHYTIQLFNHSALTPKLGVLESVFVTP
AHHRVHHVKDMAYSNKNFGGSFIFWDKLFGTFCPSLPQAPFDYGVDGAKPSANPFWASNL
PLLRYMHMPVSVSDEGGRWRCSNLTVFSGALLVFVGVIAYVYEYGYGYTNVHWPQVALFT
LLALGPVALAGMVQGRAWSVMGWLAISLGLPMLFLGLLRWSQGYWQVVLVSLGVHGVAVL
WSCTRRRSDAAPAVEKSHG