Protein Info for AO353_10270 in Pseudomonas fluorescens FW300-N2E3

Annotation: glycosyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 PF13439: Glyco_transf_4" amino acids 52 to 172 (121 residues), 38.4 bits, see alignment E=3.3e-13 PF00534: Glycos_transf_1" amino acids 197 to 350 (154 residues), 107 bits, see alignment E=2.1e-34 PF20706: GT4-conflict" amino acids 197 to 308 (112 residues), 55.7 bits, see alignment E=1e-18 PF13692: Glyco_trans_1_4" amino acids 199 to 339 (141 residues), 88.7 bits, see alignment E=1.1e-28 PF13524: Glyco_trans_1_2" amino acids 282 to 363 (82 residues), 25.8 bits, see alignment E=2.6e-09

Best Hits

KEGG orthology group: K12994, alpha-1,3-rhamnosyltransferase [EC: 2.4.1.-] (inferred from 77% identity to pfo:Pfl01_5687)

Predicted SEED Role

"Glycosyltransferase (EC 2.4.1.-)" (EC 2.4.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-

Use Curated BLAST to search for 2.4.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W4X5 at UniProt or InterPro

Protein Sequence (376 amino acids)

>AO353_10270 glycosyl transferase (Pseudomonas fluorescens FW300-N2E3)
MQIALNARILQAPRTGIGHYLAELVNALTHEPDLELSFFHGWGWSSSLPAAAMPAYSRLT
PLLRQIPGAYQARRWLEQRRFDQGHSKAIDLYHEPSLWPLAFKGPTIITLHDLTHLHYPS
TQPAARLREIERRLGQGVRQARLILTDSQFVADEAQRYFGLGPERFVVAPLGAAARFHPR
SNQFLQQALHPHGVQPQGYFLCVGTLEPRKNLSLALRAHAQLPEALRQHFPLLVVGMAGW
KGEQLVNELHTALASGHVRLLGYLPDEQVAELLAGARALVFPSIYEGFGLPVLEAMASGT
PVILTRRSAMPEVAGAAGNYIEPDDLHGLSEAMRCLADDQAHWQACREAGLRQAKLFSWE
RCAAITASAYHQALGG