Protein Info for AO353_10085 in Pseudomonas fluorescens FW300-N2E3

Annotation: pyridoxamine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00687: pyridoxal kinase
  • amino acids 6 to 286 (281 residues), 357.9 bits, E=2.1e-111
• PF08543: Phos_pyr_kin
  • amino acids 74 to 257 (184 residues), 42.5 bits, E=5.2e-15
• PF00294: PfkB
  • amino acids 135 to 262 (128 residues), 29.2 bits, E=6e-11

Best Hits

Swiss-Prot: 92% identical to PDXY_PSEPF: Pyridoxal kinase PdxY (pdxY) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00868, pyridoxine kinase [EC: 2.7.1.35] (inferred from 92% identity to pfs:PFLU6081)

MetaCyc: 55% identical to pyridoxal kinase 2 (Escherichia coli K-12 substr. MG1655)
Pyridoxal kinase. [EC: 2.7.1.35]

Predicted SEED Role

"Pyridoxal kinase (EC 2.7.1.35)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.7.1.35)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
• pyridoxal 5'-phosphate salvage I (5/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (5/9 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (4/8 steps found)

KEGG Metabolic Maps

• Vitamin B6 metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.35

Sequence Analysis Tools

See A0A0N9VN84 at UniProt or InterPro

Protein Sequence (290 amino acids)

>AO353_10085 pyridoxamine kinase (Pseudomonas fluorescens FW300-N2E3)
MKRTPHLLAIQSHVVFGHAGNSAAVFPMQRVGVNVWPLNTVQFSNHTQYGQWAGEVLAPQ
QIPDLVEGIAAIGELGNCDAVLSGYLGSAAQGRAILSGVARIKAINPSALYLCDPVMGHA
EKGCIVPAEVSNFLLDEAAAVADFMCPNQLELDSFSGRKPQSLFDCLAMARALLARGPKA
VLVKHLDYPGKLPESFEMLLVTAEGSWHLRRPLLAFPRQPVGVGDLTSGLFLARVLLGDS
LVAAFEFAASAVHEVLLETQASASYELELVRAQDRIAHPRVKFEAMAISL