Protein Info for AO353_09955 in Pseudomonas fluorescens FW300-N2E3

Annotation: sodium:alanine symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 59 to 83 (25 residues), see Phobius details amino acids 89 to 109 (21 residues), see Phobius details amino acids 138 to 158 (21 residues), see Phobius details amino acids 171 to 193 (23 residues), see Phobius details amino acids 203 to 224 (22 residues), see Phobius details amino acids 233 to 255 (23 residues), see Phobius details amino acids 294 to 315 (22 residues), see Phobius details amino acids 339 to 361 (23 residues), see Phobius details amino acids 378 to 403 (26 residues), see Phobius details amino acids 407 to 427 (21 residues), see Phobius details TIGR00835: amino acid carrier protein" amino acids 13 to 432 (420 residues), 471.1 bits, see alignment E=1.6e-145 PF01235: Na_Ala_symp" amino acids 47 to 445 (399 residues), 513 bits, see alignment E=3.6e-158

Best Hits

Swiss-Prot: 48% identical to GLNT_BACSU: Probable sodium/glutamine symporter GlnT (glnT) from Bacillus subtilis (strain 168)

KEGG orthology group: K03310, alanine or glycine:cation symporter, AGCS family (inferred from 91% identity to pba:PSEBR_a108)

Predicted SEED Role

"sodium/alanine transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WTU2 at UniProt or InterPro

Protein Sequence (483 amino acids)

>AO353_09955 sodium:alanine symporter (Pseudomonas fluorescens FW300-N2E3)
MLEVINDFLSGKVLIVLIVGLGSYFTIRSRFVQLRHFLHMFSVFKESLRSSSEQLSSFQA
LMLSLAGRVGAGNIAGVGIAVTLGGPGAVFWMWVTALVGMSSSFFECSLGQLYKRCDSEG
QYRGGPSYYIQHGLQKRWLGMIMAFLLLVTFGFAFNGLQSHAVTHSLNNAFGLNTTYTGL
GLAVLLGLVFIGGIKRIAKVADLLVPVKTLVYIGVTIYVIVLQFDHVPGMLMTIVKSAFG
LDQAFGGLIGSAIVMGVKRGVFANEAGLGSAPNVAAVASVEHPVAQGVVQAFSVFLDTFV
ICTCTALLILLSGFYTPGFEGDGIALTQNSLAAVVGDWGRMFISVALALFVFTSILYNYY
LGESNLRFLVGENRKVLIGYRALVLALIFWGAIENLSTVFAFADITMTMLAFVNLIALFL
LFNVGMRILNDYDAQRRAGIKTPVFDSSKFPDLDLDLKAWPANPPAAAPKSEAEPQGVTA
AQR