Protein Info for AO353_09905 in Pseudomonas fluorescens FW300-N2E3

Annotation: phosphate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR02136: phosphate binding protein" amino acids 3 to 310 (308 residues), 326.6 bits, see alignment E=6.9e-102 PF12849: PBP_like_2" amino acids 38 to 296 (259 residues), 130.2 bits, see alignment E=1.3e-41 PF13531: SBP_bac_11" amino acids 48 to 307 (260 residues), 27.5 bits, see alignment E=2.6e-10

Best Hits

Swiss-Prot: 84% identical to PSTS_PSEAE: Phosphate-binding protein PstS (pstS) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02040, phosphate transport system substrate-binding protein (inferred from 98% identity to pfo:Pfl01_5615)

Predicted SEED Role

"Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WIG1 at UniProt or InterPro

Protein Sequence (334 amino acids)

>AO353_09905 phosphate-binding protein (Pseudomonas fluorescens FW300-N2E3)
MKLKRLMAAMTFVAAGVATANAVAAGVDPAIPAYTKATGVSGNLSSVGSDTLANLMTLWA
ENYKKEYPNVNIQIQAAGSATAPPALTEGTSNLGPMSRKMKDTELAAFEQKYGYKPTAIP
VAVDALAVFVHKDNPIQHLTMEQVDAIFSSTRLCGAKAEVKTWGDLGVTGDLANKPVQLF
GRNSVSGTYGYFKEEALCKGDYKPNVNEQPGSASVVQSISSSLNGIGYSGIGYKTASVKT
VALAKKGSTDYIADSEENALNGKYPLSRFLYVYVNKAPNKPLAPLEAEFVKLVLSKQGQE
VVVKDGYIPLPAKVAAKALADLGLQEGGAEVAKK