Protein Info for AO353_09830 in Pseudomonas fluorescens FW300-N2E3

Annotation: pyridine nucleotide-disulfide oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 PF07992: Pyr_redox_2" amino acids 5 to 282 (278 residues), 168.8 bits, see alignment E=4.7e-53 PF00070: Pyr_redox" amino acids 145 to 218 (74 residues), 35.4 bits, see alignment E=3.2e-12 PF18113: Rbx_binding" amino acids 307 to 377 (71 residues), 90.7 bits, see alignment E=1e-29

Best Hits

Swiss-Prot: 80% identical to RURE_PSEAE: Rubredoxin-NAD(+) reductase (alkT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K05297, rubredoxin-NAD+ reductase [EC: 1.18.1.1] (inferred from 94% identity to pba:PSEBR_a133)

Predicted SEED Role

"Rubredoxin-NAD(+) reductase (EC 1.18.1.1)" in subsystem Rubrerythrin (EC 1.18.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.18.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WGM2 at UniProt or InterPro

Protein Sequence (382 amino acids)

>AO353_09830 pyridine nucleotide-disulfide oxidoreductase (Pseudomonas fluorescens FW300-N2E3)
MNAPVVIVGTGLAGYNLAREFRKLDGETPLLLITADDGRSYSKPMLSTGFGKNKDADGLS
MAEPGAMAEQLKAEVRTHTRISGIDPGHKRLWIGEEAVAYRDLILAWGAETVRVPVQGDA
PEAIFPINDLEDYARFRAAAAGKRRVLLLGAGLIGCEFANDLILGGYEVQLVAPCEQVMP
TLLHPAAAAAVQAGLESLGAQFHLGPVLNSLQRTDDGLEGHLSDGQVIPCDVVVSAIGLR
PRIDLAAAAGLQINRGVVVDRHLKTSHANIYALGDCAEVDGLNLLYVMPLMSCARALAQT
LAGNPTAVSYGAMPITVKTPVCPLVVSPPPRGFEGAWTVEGQGADIKALCRDASGKLLGY
ALTGAAVMEKLALNKELPALLA