Protein Info for AO353_09655 in Pseudomonas fluorescens FW300-N2E3

Annotation: type VI secretion protein ImpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 TIGR03361: type VI secretion system Vgr family protein" amino acids 11 to 523 (513 residues), 679.4 bits, see alignment E=3e-208 TIGR01646: Rhs element Vgr protein" amino acids 24 to 506 (483 residues), 625.1 bits, see alignment E=9.3e-192 PF05954: Phage_GPD" amino acids 30 to 330 (301 residues), 366 bits, see alignment E=1.5e-113 PF04717: Phage_base_V" amino acids 386 to 453 (68 residues), 52.8 bits, see alignment E=4.1e-18

Best Hits

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a5567)

Predicted SEED Role

"VgrG-3 protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VTM9 at UniProt or InterPro

Protein Sequence (645 amino acids)

>AO353_09655 type VI secretion protein ImpA (Pseudomonas fluorescens FW300-N2E3)
MLFNQASRLAKVTSPLGPEVLLLKDMGGGEELGRLFNYELQLHSLDNAIDLNQLLGKPMS
LSLQLDGGGERHFHGIVARCSQNVDQGQFASYQVTLRPWLWLLTRTSDCRIFQNLSIPQI
IKQVFRDLGFSDFEDALSRPYREWEYCVQYRETSFDFVSRLMEQEGIYYFFRHEQGRHVL
VLADAYGAHTSAPGYASVPYYPRNEQQRERDHIHDWHLAQEVQPGSLELNDYDFQRPSAR
IDVRSAMPRPHTAGDYPLYDYPGTYVQSEDGEHYARTRIEALQTLHEQVELAGNARGLGS
GHLFSLTGFSRQDQNREYLIVGTRYYISQESGETGGGAPSAQFESSLTCIDAQQSYRPLP
NTHRPIVKGPQTALVVGPKGEEIWTDQFGRVKVHFYWDRHDQSNENSSCWIRVSQSWAGK
NWGSMQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQTVPYDLPENATQSGMKSRSSKGG
TPANFNEIRMEDKKGAEQLYIHAERNQDIVVEVDESHSVGHDRNKSIGHNETVTIGNNRL
RIVKQEDILSVGQRKTDSISQSYVIEVGENLRLVCGESILELNASGQINLTGVQISFYAS
GDAEFNTGGVLHLNNGGGAGATPDGQGVKASIDANINAAFPKSKG