Protein Info for AO353_09455 in Pseudomonas fluorescens FW300-N2E3

Annotation: type VI secretion protein ImpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 TIGR03362: type VI secretion-associated protein, VC_A0119 family" amino acids 12 to 484 (473 residues), 435.1 bits, see alignment E=2e-134 PF06812: ImpA_N" amino acids 18 to 127 (110 residues), 102.8 bits, see alignment E=1.3e-33 PF16989: T6SS_VasJ" amino acids 225 to 483 (259 residues), 260.2 bits, see alignment E=2.1e-81

Best Hits

KEGG orthology group: K11910, type VI secretion system protein VasJ (inferred from 87% identity to pst:PSPTO_5434)

Predicted SEED Role

"Uncharacterized protein ImpA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZU1 at UniProt or InterPro

Protein Sequence (518 amino acids)

>AO353_09455 type VI secretion protein ImpA (Pseudomonas fluorescens FW300-N2E3)
MSYSNKLSAHYLELAKASVSKENFAGEDVRFSSEYEALESELSKAQSMHEIGQIDWLKIR
ENSESLLRAQSKDLRVGAWLTWALYQRESFQGLLAGLGFLHHLCENHWTEVHPNKARTRS
AAISWLVPRLEQVLNENVAIKEQLPLFRRLVEHLEGLDAACTEHMGDDAPLLLPISRRLK
NMVQRAADNQPEPGAVGAVVAQVKQVATQLFTPGAPIDNEKEAHKALRAQQENARPLCAW
WLKQKATDLRALRLNRTLLWLPIDAVPERNAEQITALRGLPADKLKIYQDRFDQGQFADL
LVELEASLAKAPFWFDGQRLVWECLQGLNAELAMREVEIYFALLIQRLPGIVELRYHDGA
PFADPATRAWISAHVMPHLQTASAPRKVEVSASQPAWELALEDVLPVLRKDGLKAAVQIL
KQGLQAAHGGRVRFFWQFSLARLCFLAKKYELAKTQLETLDSELENSGLHAWEPDLALEV
LHLLHSCCELLPQNHAVRERKEEIYRRLCHLDLEVVLE