Protein Info for AO353_09385 in Pseudomonas fluorescens FW300-N2E3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 transmembrane" amino acids 242 to 266 (25 residues), see Phobius details TIGR03349: type IV/VI secretion system protein, DotU family" amino acids 61 to 270 (210 residues), 248.3 bits, see alignment E=3.4e-78 PF09850: DotU" amino acids 63 to 264 (202 residues), 237.7 bits, see alignment E=4.9e-75

Best Hits

KEGG orthology group: K11892, type VI secretion system protein ImpK (inferred from 92% identity to pfo:Pfl01_5578)

Predicted SEED Role

"Outer membrane protein ImpK/VasF, OmpA/MotB domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WH65 at UniProt or InterPro

Protein Sequence (291 amino acids)

>AO353_09385 hypothetical protein (Pseudomonas fluorescens FW300-N2E3)
MIKEMDHSQDDKTVLLDRQGHGPAQSPLTDFAAPPRFEQLEERMIYAARLRPAEAFNISL
NSLVAASSDLLSEVVRLKHSATREDMHALNQRLTAALKLFEVRALHNGAESSQVMAARYV
LCTVVDEAVVTTPWGNESEWSQMSLLSSFHNETFGGEKFFQLLDRLSKNPIKHLPMLELM
YLCLSLGFEGKYRVQARGMLELDGIRDALYRQIRQLRGDVPRELSPHWEGLNDRRRSLVR
IVPWWMVALFTLVCLVVMYSGFAWVLGEQRDTVLQPYQPLDPAAVQPLSKP