Protein Info for AO353_08980 in Pseudomonas fluorescens FW300-N2E3
Annotation: heme biosynthesis operon protein HemX
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02496, uroporphyrin-III C-methyltransferase [EC: 2.1.1.107] (inferred from 82% identity to pfl:PFL_6000)Predicted SEED Role
"Uroporphyrinogen-III synthase (EC 4.2.1.75)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 4.2.1.75)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
- cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (13/13 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
- cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)
- superpathway of heme b biosynthesis from glutamate (9/10 steps found)
- tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
- siroheme biosynthesis (4/4 steps found)
- superpathway of heme b biosynthesis from glycine (6/8 steps found)
- tetrapyrrole biosynthesis II (from glycine) (3/4 steps found)
- factor 430 biosynthesis (3/7 steps found)
- superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.107, 4.2.1.75
Use Curated BLAST to search for 2.1.1.107 or 4.2.1.75
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9VSM0 at UniProt or InterPro
Protein Sequence (374 amino acids)
>AO353_08980 heme biosynthesis operon protein HemX (Pseudomonas fluorescens FW300-N2E3) VSETALPKDNVQPALDASVETVKSPEPRRGSGLVILAMLLGAAGVAAGGWGIWQVRSLQA NNQQQLGQLQALNDQAQSLKLNEQRLSARLEQLPPANELEDRRRLVIQLQGDQQRLNQRL ETVLGASRKDWRLAEAEHLLRLASLRLSALQDIGSAQALVQGADEILREQNDPGSFAARE QIAKSLAALRSTEQPDRTGLFLQLGALRDQVAELSELAPEFHERDSLSALTSDGDGASRW AQWWDQISRYIRIDFNADKNIRPLLAGQSLTQVRLALSLALEQAQWAALNGQAPVYTQAL TEARDVLKGSFNQDNPQSKVMLEQVAELSKQPVTVITPDLATTLSTVQAYLERRNVSAED SVKPAASTTPVTSP