Protein Info for AO353_08885 in Pseudomonas fluorescens FW300-N2E3

Annotation: polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 737 transmembrane" amino acids 465 to 481 (17 residues), see Phobius details PF13089: PP_kinase_N" amino acids 54 to 159 (106 residues), 122.7 bits, see alignment E=2.1e-39 TIGR03705: polyphosphate kinase 1" amino acids 54 to 727 (674 residues), 942.7 bits, see alignment E=6.3e-288 PF02503: PP_kinase" amino acids 170 to 351 (182 residues), 207.8 bits, see alignment E=2.8e-65 PF17941: PP_kinase_C_1" amino acids 378 to 541 (164 residues), 233.3 bits, see alignment E=3.1e-73 PF13090: PP_kinase_C" amino acids 550 to 717 (168 residues), 217.1 bits, see alignment E=2.8e-68

Best Hits

Swiss-Prot: 92% identical to PPK1_PSESM: Polyphosphate kinase (ppk) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 95% identity to pfo:Pfl01_5464)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WI19 at UniProt or InterPro

Protein Sequence (737 amino acids)

>AO353_08885 polyphosphate kinase (Pseudomonas fluorescens FW300-N2E3)
MNTEGLTEVAVKEAQPLVEPVVEIEPAPPAVVVAEAAPVAPAVAIPGLDDSSLYIHRELS
QLQFNIRVLEQALDESYPLLERLKFLLIFSSNLDEFFEIRVAGLKKQITFAREQAGADGL
QPHQALARISDLVHGHVDRQYAILNDILLPELEKHQVRFIRRRYWTTKLKTWVRRYFRDE
IAPIITPIGLDPTHPFPLLVNKSLNFIVELEGIDAFGRDSGLAIIPAPRLLPRVIKVPEE
VGGTGDNYVFLSSMIHAHADDLFQGMKVKGCYQFRLTRNADLAVDTEDVEDLARALRGEL
FSRRYGDAVRLEVADTCPKHLSDYLLKQFNLSETELYQVNGPVNLTRLFSITGLDSHPEL
QYTPFTPQIPKLLQNSENIFSVISKQDILLLHPFESFTPVVDLLRQAAKDPHVLAVRQTL
YRSGANSEIVDALVDAARNGKEVTAVIELRARFDEESNLQLASRLQAAGAVVIYGVVGFK
THAKMMLILRREAGEIVRYAHLGTGNYHAANARLYTDYSLLTSDDALCEDVGKLFSQLIG
MGKTLRMKKLLHAPFTLKKGMLDMIARETQFALDGKPAHIIAKFNSLTDPKVIRALYKAS
QSGVRIDLVIRGMCCLRPGIAGVSHNIHVRSIIGRFLEHTRVFYFLNGGEEQMFLSSADW
MERNLDKRVETCFPVEGKKLITRVKKELELYLTDNTHSWSLQPDGRYIRNTPTGNQNPRS
AQATLLERLGSPILTVN