Protein Info for AO353_08720 in Pseudomonas fluorescens FW300-N2E3

Annotation: glycine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 957 PF02347: GDC-P" amino acids 18 to 441 (424 residues), 590 bits, see alignment E=2.9e-181 amino acids 461 to 729 (269 residues), 28.4 bits, see alignment E=8.1e-11 TIGR00461: glycine dehydrogenase" amino acids 19 to 950 (932 residues), 1536.6 bits, see alignment E=0 PF21478: GcvP2_C" amino acids 779 to 900 (122 residues), 196.2 bits, see alignment E=1.5e-62

Best Hits

Swiss-Prot: 93% identical to GCSP2_PSEPF: Glycine dehydrogenase (decarboxylating) 2 (gcvP2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 82% identity to avn:Avin_47260)

MetaCyc: 68% identical to glycine decarboxylase (Escherichia coli K-12 substr. MG1655)
Glycine dehydrogenase (decarboxylating). [EC: 1.4.1.27, 1.4.4.2]

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.4.2

Use Curated BLAST to search for 1.4.1.27 or 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZS3 at UniProt or InterPro

Protein Sequence (957 amino acids)

>AO353_08720 glycine dehydrogenase (Pseudomonas fluorescens FW300-N2E3)
MSQLPSLSQLRDPNAFLRRHLGPDTAEQQAMLDSLGLGSRVELIEQTVPPGIRLNRVLDL
PPALDEEAALAKLRGYAEQNQVWTSLIGMGYHGTLTPAVILRNVLENPGWYTAYTPYQPE
IAQGRLEALLNFQQLTIDLTGLELANASLLDEATAAAEAMALAKRVAKSKSNLFFVDENC
HPQTISVVKTRAEGFGFELIVDTVDNLKQHQVFGALLQYPDTHGDIRDLRPLIDHLHAQQ
ALACVAADLLSLLLLTPPGEMGADVVFGSSQRFGVPMGYGGPHAAFFASRDEFKRAIPGR
IIGVSKDARGNVALRMALQTREQHIRREKANSNICTAQVLLANIASFYAVYHGPQGLKRI
AQRVHRLTCILAAGLERNGITRLNQHFFDTLTLEVGGSQTAIIDSAKAAQINLRILGRGQ
VGLSLDESCDERTVAKLFDVFLGADHGLDVAELDGEPLVSGIPESLLRTTSYLRHPVFNA
HHSETEMLRYLKQLENKDLALNQSMIPLGSCTMKLNASSEMIPITWPAFANLHPFAPKEQ
AVGYSLMISELESWLCAITGFDAICMQPNSGAQGEYAGLLAIRKYHESRQQGARDICLIP
ASAHGTNPASAQMAGMRVMIVDCDDAGNVDLDDLKAKATAAGDKLSCLMATYPSTHGVYE
EGISEICEVIHSHGGQVYMDGANLNAQVGLARPADIGADVSHMNLHKTFCIPHGGGGPGM
GPIGVRAHLAPFVANHPVVPIEGPHPQNGAVSAAPWGSASILPISWMYIAMMGPQLADAS
EVAILSANYLAKHLSGAFPVLYTGRNERVAHECILDLRPLKAQTGISEEDVAKRLMDYGF
HAPTMSFPVPGTLMVEPTESESKAELDRFISAMLSIRAEIAEVQNGNWSAEDNPLKRSPH
TLADITGVWDRPYSIEQAVTPDAHTRLHKYWPTVNRVDNVYGDRNLFCACVPVDEYR