Protein Info for AO353_08595 in Pseudomonas fluorescens FW300-N2E3

Updated annotation (from data): Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)
Rationale: Specifically important for: Potassium acetate; L-Arginine; Putrescine Dihydrochloride; L-Citrulline. This is the first step in putrescine degradation and is important for the usage of putrescine as either C or N source. The phenotypes on citrulline and arginine might indicate a side pathway where they are converted to putrescine via ornithine decarboxylase (SEED_correct)
Original annotation: gamma-glutamylputrescine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00120: Gln-synt_C
  • amino acids 121 to 454 (334 residues), 370.5 bits, E=4e-115

Best Hits

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 98% identity to pfs:PFLU5849)

Predicted SEED Role

"Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)" (EC 6.3.1.11)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• superpathway of ornithine degradation (7/8 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• ammonia assimilation cycle II (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• putrescine degradation II (3/4 steps found)
• L-aspartate degradation II (aerobic) (2/3 steps found)
• L-aspartate degradation III (anaerobic) (2/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.11, 6.3.1.2

Use Curated BLAST to search for 6.3.1.11 or 6.3.1.2

Sequence Analysis Tools

See A0A0N9VMN1 at UniProt or InterPro

Protein Sequence (458 amino acids)

>AO353_08595 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (Pseudomonas fluorescens FW300-N2E3)
MSVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
LFALDINGSTVESTGLGLDIGDADRICYPIPNTLCNEPWQKRPTAQLLMTMHELEGEPFF
ADPREVLANVVRKFDDMGLTICAAFELEFYLIDQENVNGRPQPPRSPVSGKRPHSTQVYL
IDDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPIKACDYAVLLKRL
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISILDKDGKNIFASEDPEQNAALRHAIGGV
LETLPAQMAFLCPNVNSYRRFGAQFYVPNSPCWGLDNRTVAIRVPTGSADAVRIEHRVAG
ADANPYLLMASVLAGVHHGLTNKIEPGAPVEGNSYEQNEQSLPNNLRDALRELDDSEVMA
KYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV