Protein Info for AO353_08585 in Pseudomonas fluorescens FW300-N2E3
Updated annotation (from data): Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)
Rationale: Specifically important for: Putrescine Dihydrochloride. KEGG annotates this as putrescine aminotransferase, probably based on similarity to spuC (PA0299) of P. aeruginosa, but it is believed that only the g-glutamyl- pathway operates in P. aeruginosa (X. Yao et al 2011, PMC:3147493)
Original annotation: aminotransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 78% identical to SPUC_PSEAE: Putrescine--pyruvate aminotransferase (spuC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K12256, putrescine aminotransferase [EC: 2.6.1.-] (inferred from 93% identity to pfl:PFL_5927)MetaCyc: 78% identical to putrescine--pyruvate aminotransferase (Pseudomonas aeruginosa)
RXN-8 [EC: 2.6.1.113]
Predicted SEED Role
"Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18)" in subsystem Pyruvate Alanine Serine Interconversions (EC 2.6.1.18)
MetaCyc Pathways
- superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
- superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
- superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
- β-alanine biosynthesis II (5/6 steps found)
- β-alanine degradation II (2/2 steps found)
- 4-aminobutanoate degradation II (2/2 steps found)
- 4-aminobutanoate degradation III (2/2 steps found)
- putrescine degradation V (2/2 steps found)
- superpathway of 4-aminobutanoate degradation (2/3 steps found)
- 4-aminobutanoate degradation I (1/2 steps found)
- GABA shunt II (2/4 steps found)
- GABA shunt I (1/4 steps found)
- 4-aminobutanoate degradation V (2/7 steps found)
- nicotine degradation I (pyridine pathway) (6/17 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Aminophosphonate metabolism
- Arginine and proline metabolism
- Butanoate metabolism
- Caprolactam degradation
- Glutamate metabolism
- Lysine biosynthesis
- Lysine degradation
- Methionine metabolism
- Nucleotide sugars metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.-, 2.6.1.18, 2.6.1.19
Use Curated BLAST to search for 2.6.1.- or 2.6.1.113 or 2.6.1.18 or 2.6.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A0N9VTA1 at UniProt or InterPro
Protein Sequence (454 amino acids)
>AO353_08585 Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) (Pseudomonas fluorescens FW300-N2E3) MTSKNPQTREWQTLSSEHHLAPFSDFKQLKEKGPRIITNAKGVYLWDSEGNKILDGMAGL WCVAIGYGRDELADAASKQMRELPYYNLFFQTAHPPVLELAKAISDIAPEGMNHVFFTGS GSEGNDTMLRMVRHYWAIKGQPNKKVIISRINGYHGSTVAGASLGGMTYMHEQGDLPIPG IVHIPQPYWFGEGGDMTPEEFGIWAANQLEEKILELGVDTVGAFIAEPIQGAGGVIIPPD SYWPRIKEILAKYDILFVADEVICGFGRTGEWFGSDFYGLKPDMMTIAKGLTSGYIPMGG LIVRDEVVEVLNEGGDFNHGFTYSGHPVAAAVALENIRILREEKIIEHVRAETAPYLQKR LRELNDHPLVGEVRGVGLLGAIELVQDKATRARYVGKGVGMICRQFCFDNGLIMRAVGDT MIIAPPLVITKAEIDELVTKARKCLDLTLSALQS