Protein Info for AO353_08580 in Pseudomonas fluorescens FW300-N2E3

Annotation: spermidine/putrescine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF01547: SBP_bac_1" amino acids 40 to 299 (260 residues), 77.5 bits, see alignment E=4.9e-25 PF13531: SBP_bac_11" amino acids 41 to 299 (259 residues), 36.2 bits, see alignment E=1.4e-12 PF13416: SBP_bac_8" amino acids 42 to 329 (288 residues), 106.5 bits, see alignment E=5.6e-34 PF13343: SBP_bac_6" amino acids 75 to 317 (243 residues), 71.8 bits, see alignment E=1.6e-23

Best Hits

Swiss-Prot: 73% identical to SPUD_PSEAE: Putrescine-binding periplasmic protein SpuD (spuD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 90% identity to pfs:PFLU5845)

MetaCyc: 57% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W1K8 at UniProt or InterPro

Protein Sequence (364 amino acids)

>AO353_08580 spermidine/putrescine ABC transporter substrate-binding protein (Pseudomonas fluorescens FW300-N2E3)
LKKTGKTLLALSLMGVMAGAAQADDKVLHVYNWSDYIAPDTIANFEKESGIKVVYDVFDS
NETLEAKLLAGKSGYDVVVPSNNFLAKQIKAGVYQELDKSKLTNYGNLNKSLLKAVSVSD
PDNKHAFPYMWGSIGIGYNAEKVKAALGIDKIDSWDVVLKPENIAKLKSCGVSFLDSPTE
MLPVALHYLGLPTDSQKKEDLKKAEELFLKIRPSITYFHSSKYISDLANGNICVAVGYSG
DVQQAKTRAAEAGDKVKVSYAIPKEGAGSFYDMVAIPKDAENVEGAYKFMNYLLQPKVMA
EITNAVRFPNGNAEATQYVNKDITSDPGIYPTADVQSKLYAIADLPAATQRELTRSWTKI
KSGK