Protein Info for AO353_08445 in Pseudomonas fluorescens FW300-N2E3

Annotation: thymidylate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03284: thymidylate synthase
  • amino acids 2 to 323 (322 residues), 204.6 bits, E=9.8e-65
• PF00303: Thymidylat_synt
  • amino acids 2 to 323 (322 residues), 291.8 bits, E=2e-91

Best Hits

Swiss-Prot: 90% identical to TYSY_PSESM: Thymidylate synthase (thyA) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00560, thymidylate synthase [EC: 2.1.1.45] (inferred from 96% identity to pba:PSEBR_a242)

Predicted SEED Role

"Thymidylate synthase (EC 2.1.1.45)" in subsystem Folate Biosynthesis (EC 2.1.1.45)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (5/9 steps found)
• pyrimidine deoxyribonucleosides salvage (1/5 steps found)

KEGG Metabolic Maps

• One carbon pool by folate
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.45

Sequence Analysis Tools

See A0A0N9VSF2 at UniProt or InterPro

Protein Sequence (323 amino acids)

>AO353_08445 thymidylate synthase (Pseudomonas fluorescens FW300-N2E3)
MKQYLDLVAHVIKNGTKQANRTGVNTISFPGAMLRYDLKEGFPAITTRKMAFKSAIGEMV
GFLRGVNNAAEFRALGCKVWDQNANENAQWLANPFRQGEDDLGEIYGVQWRKWPAYKQIP
VSNQAAIEQTLSQGYRQIAEGEEDGQAYVVLYKAIDQIRQCVDTIIKDPGSRRILFHGWN
CAQLDEMALPPCHLLYQFHPNVETKEISLTLYIRSNDLGLGTPFNLTEGAALLSLIGRLT
GYTPRWFTYFIGDAHVYENHLDMLNEQLKREPFPMPKLVISDRVPEFAKTGVYQPEWLEQ
IEPSDFSLEGYEHHAPMTAPMAV