Protein Info for AO353_08420 in Pseudomonas fluorescens FW300-N2E3

Annotation: ATPase P

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 763 transmembrane" amino acids 149 to 167 (19 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 204 to 220 (17 residues), see Phobius details amino acids 226 to 244 (19 residues), see Phobius details amino acids 377 to 402 (26 residues), see Phobius details amino acids 408 to 434 (27 residues), see Phobius details amino acids 714 to 734 (21 residues), see Phobius details amino acids 740 to 761 (22 residues), see Phobius details TIGR01511: copper-translocating P-type ATPase" amino acids 191 to 760 (570 residues), 397.4 bits, see alignment E=2.4e-122 TIGR01512: cadmium-translocating P-type ATPase" amino acids 206 to 762 (557 residues), 472.9 bits, see alignment E=2.4e-145 TIGR01525: heavy metal translocating P-type ATPase" amino acids 206 to 760 (555 residues), 587.5 bits, see alignment E=6.8e-180 TIGR01494: HAD ATPase, P-type, family IC" amino acids 228 to 743 (516 residues), 269.6 bits, see alignment E=8.1e-84 PF00122: E1-E2_ATPase" amino acids 257 to 438 (182 residues), 193.1 bits, see alignment E=4.8e-61 PF00702: Hydrolase" amino acids 457 to 670 (214 residues), 124.3 bits, see alignment E=1.3e-39

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 85% identity to pfl:PFL_5892)

MetaCyc: 76% identical to cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
3.6.3.3-RXN [EC: 7.2.2.21]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9VMJ8 at UniProt or InterPro

Protein Sequence (763 amino acids)

>AO353_08420 ATPase P (Pseudomonas fluorescens FW300-N2E3)
MSDSQHTHKPHADHDHDHDHDHDHSHGQKLQPVQKHDHASGGGSCCSSAAAPSLIKLSET
PTDGARLSRFRIDAMDCPTEQTLIQNKLGKLAGVQQLEFNLINRVLGVTHDLPTTAPIID
AIKSIGMLAEPIEQGADAQASPAPVKKPWWPLAVSGVGALAAEVIHFTSAAPTWVVAIVA
LISILSGGLGTYKKGWIALKNLNLNINALMSIAVTGAVLIGQWPEAAMVMFLFTVAELIE
AKSLDRARNAISGLMQMTPEKVTVQQEDGSWVEREAKSIDLGARVRVRPGERIGLDGEVV
SGSSTIDQAPITGESLPVEKTVGDKVFAGTINQSGSLEFKVTAAADNSTLARIIHAVEAA
QGARAPTQRFVDRFSKIYTPVVFILALAVALIAPLFMGAAWFDWIYRALVLLVVACPCAL
VISTPVTIVSGLAAAARKGILIKGGVYLEGGHELDYLALDKTGTITHGKPVQTDYLSLDP
TVESTAQILAASLAGRSDHPVSLAIANAAVDKQLTLLAVDNFEALTGRGVRGEIDGQVYH
LGNHRLVEELGLCSPALEEKLFALEEQGKTVVLLLDRSGPLALFAVADTVKASSREAIRQ
LHELGIKTLMLTGDNPHTARAIAAQVGIDEARGDLLPTDKLKAIEGLYAKGHRVGMVGDG
INDAPALARAQIGFAMAAAGTDTAIETADVALMDDDLRKIPAFIRLSRQTSNILTQNIAL
ALIIKAIFLGVTFVGLATMWMAVFADMGVALLVVFNGLRLLRK