Protein Info for AO353_08355 in Pseudomonas fluorescens FW300-N2E3

Annotation: GDSL family lipase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 transmembrane" amino acids 14 to 33 (20 residues), see Phobius details PF04311: DUF459" amino acids 110 to 356 (247 residues), 38.4 bits, see alignment E=9e-14 PF13472: Lipase_GDSL_2" amino acids 159 to 347 (189 residues), 42.1 bits, see alignment E=1.4e-14

Best Hits

Predicted SEED Role

"putative periplasmic protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZR4 at UniProt or InterPro

Protein Sequence (366 amino acids)

>AO353_08355 GDSL family lipase (Pseudomonas fluorescens FW300-N2E3)
MPVSNASPLPTNLSGALKVLYAILVISALLFWFNQDSIRLYCQQKYHDGCEIPGLAQNPQ
WRFGAQLNQTLADVRSGFIARLEGLDDVKLIDQAQAAVNEEEPLGETLAPLPTLEQAAKS
VEAPPQVVTDPAVKPAPPVDGAPVTPLMATLALGDEVLFVGDSLMQGVAPHMANTLLKRY
KVKSINLSRQSTGLAYPSFFNWPKTVESTLQGHPKIRLMVMFLGPNDPWDMPDGKHKPFL
RFKTPEWEVAYRQRIDSILDSARTHQVQVIWVGPPNMEKAKLSTAMAYLNGLYKSQLAAY
HQHFVSANEILGYQTDEFSYYLTVDGSRKVKTRVDDGIHFTPTGQRLIAERVLSLINFPS
QQLTEH