Protein Info for AO353_08320 in Pseudomonas fluorescens FW300-N2E3

Annotation: 5-hydroxymethyluracil DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 270 PF01149: Fapy_DNA_glyco" amino acids 1 to 115 (115 residues), 126.9 bits, see alignment E=9.3e-41 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 1 to 269 (269 residues), 292.5 bits, see alignment E=1.5e-91 PF06831: H2TH" amino acids 130 to 220 (91 residues), 83.1 bits, see alignment E=1.7e-27 PF06827: zf-FPG_IleRS" amino acids 242 to 270 (29 residues), 32.8 bits, see alignment (E = 7.1e-12)

Best Hits

Swiss-Prot: 96% identical to FPG_PSEF5: Formamidopyrimidine-DNA glycosylase (mutM) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 96% identity to pfo:Pfl01_5351)

MetaCyc: 55% identical to DNA-formamidopyrimidine glycosylase (Escherichia coli K-12 substr. MG1655)
DNA-formamidopyrimidine glycosylase. [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; RXN-21279 [EC: 3.2.2.23]

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W402 at UniProt or InterPro

Protein Sequence (270 amino acids)

>AO353_08320 5-hydroxymethyluracil DNA glycosylase (Pseudomonas fluorescens FW300-N2E3)
MPELPEVETTRRGIAPFLEGQRVSRVIVRDRRLRWPIPEDLDVRLSGQRIVLVERRAKYL
LINAEVGTLISHLGMSGNLRLVEVGLPALKHEHVDIELESGLALRYTDPRRFGAMLWSLD
PLNHELLVRLGPEPLTDLFDGERLFQLSRGRSMAVKPFIMDNAVVVGVGNIYATEALFAA
GIDPRREAKGISRARYLKLAIEIKRILAAAIERGGTTLRDFIGGDGQPGYFQQELFVYGR
GGEQCKVCGSELREVRLGQRASVFCPRCQS