Protein Info for AO353_08235 in Pseudomonas fluorescens FW300-N2E3

Annotation: RNA polymerase subunit sigma-70

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 TIGR02392: alternative sigma factor RpoH" amino acids 16 to 283 (268 residues), 410.7 bits, see alignment E=3.3e-127 PF00140: Sigma70_r1_2" amino acids 17 to 40 (24 residues), 25.1 bits, see alignment (E = 2.2e-09) TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 50 to 280 (231 residues), 101.7 bits, see alignment E=3.3e-33 PF04542: Sigma70_r2" amino acids 54 to 123 (70 residues), 64.2 bits, see alignment E=1.1e-21 PF04545: Sigma70_r4" amino acids 229 to 279 (51 residues), 56.3 bits, see alignment 2.7e-19

Best Hits

Swiss-Prot: 90% identical to RPOH_PSEAE: RNA polymerase sigma factor RpoH (rpoH) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03089, RNA polymerase sigma-32 factor (inferred from 99% identity to pfs:PFLU5777)

MetaCyc: 59% identical to RNA polymerase sigma factor RpoH (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"RNA polymerase sigma factor RpoH" in subsystem Heat shock dnaK gene cluster extended or Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7GZR1 at UniProt or InterPro

Protein Sequence (284 amino acids)

>AO353_08235 RNA polymerase subunit sigma-70 (Pseudomonas fluorescens FW300-N2E3)
MTTSLQPAYALVPGANLEAYVHTVNSIPLLTPEQERELAESLYYEQDLGAARQMVLAHLR
FVVHIARSYSGYGLAQADLIQEGNVGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEFILR
NWRIVKVATTKAQRKLFFNLRSQKKRLAWLNNEEVHRVAESLGVEPREVREMESRLTGHD
MAFDPAAEADDDSAFQSPANYLEDHRYDPARQLEDADWTDNSTHNLHEALEVLDDRSRDI
LYQRWLAEEKATLHDLAQKYNVSAERIRQLEKSAMNKLKLSIAA