Protein Info for AO353_08200 in Pseudomonas fluorescens FW300-N2E3

Annotation: coproporphyrinogen III oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 TIGR00539: putative oxygen-independent coproporphyrinogen III oxidase" amino acids 29 to 375 (347 residues), 403.7 bits, see alignment E=3.7e-125 PF04055: Radical_SAM" amino acids 31 to 200 (170 residues), 92.4 bits, see alignment E=3.8e-30 PF06969: HemN_C" amino acids 325 to 390 (66 residues), 43.9 bits, see alignment E=2e-15

Best Hits

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 93% identity to pba:PSEBR_a5329)

Predicted SEED Role

"Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB" in subsystem Heat shock dnaK gene cluster extended or Heme and Siroheme Biosynthesis or Queuosine-Archaeosine Biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W3Y3 at UniProt or InterPro

Protein Sequence (400 amino acids)

>AO353_08200 coproporphyrinogen III oxidase (Pseudomonas fluorescens FW300-N2E3)
MTHDSSASPLIHGGAQSPRAALPVLPPLALYIHIPWCVRKCPYCDFNSHTASPVLPEEEY
VDALLADLDQDLHAVYGRELSSIFFGGGTPSLFSAQALGRLLKGVEQRIPFAGDIEITLE
ANPGTFEQEKFVAYRALGINRLSIGIQSFQQEKLKALGRIHNGDEAIRAAGMARQAGFDN
FNLDLMHGLPDQSLEDALDDLRQAIALKPTHLSWYQLTLEPNTVFWNQPPTLPEDDTLWD
IQEAGQALLAEHGYAQYEVSAYAQPGRPARHNLNYWSFGDFIGIGAGAHGKLSHPDGRIV
RTWKTRLPKDYLNPAKSFQAGEKALTNEELPFEFLMNALRLTEGVEARLYPERTGLTLES
LAQGRREAEQSGLLQVEPSRLAATMRGQLFLNDLLQNFLT